Rozek, Charles E. and Davidson, Norman (1986) Differential Processing of RNA Transcribed from the Single-Copy Drosophila Myosin Heavy Chain Gene Produces Four mRNAs That Encode Two Polypeptides. Proceedings of the National Academy of Sciences of the United States of America, 83 (7). pp. 2128-2132. ISSN 0027-8424 http://resolver.caltech.edu/CaltechAUTHORS:ROZpnas86
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We report the sequence of genomic DNA at the 3' end of the single-copy Drosophila myosin heavy chain (MHC) gene and the structure and sequence at the 3' end of four MHC mRNAs. Two mRNAs, 7.2 kilobases (kb) and 8.0 kb in length, are expressed in all stages of development in which detectable levels of muscle-specific mRNAs accumulate. These mRNAs differ by alternate choice of two poly(A) sites within the same exon. Sequence information predicts that these two mRNAs can encode one MHC polypeptide. Two additional MHC mRNAs, 8.0 kb and 8.6 kb in length, are expressed only in late pupal and adult stages of development. These two stage-specific MHC mRNAs use the same poly(A) sites as the MHC mRNAs described above but have a different splicing pattern and thus include an additional exon. Sequence information predicts that these two stage-specific MHC mRNAs encode a second MHC polypeptide with a different COOH terminus.
|Additional Information:||Copyright © 1986 by the National Academy of Sciences Contributed by Norman Davidson, December 16, 1985 C.E.R. is indebted to Drs. Susan Celniker and Morris Burke for many helpful suggestions during the execution of experiments and preparation of the manuscript and to Margaret Chidlaw for help in preparation of the manuscript. This research has been supported by research grants to C.E.R. and N.D. from the National Institutes of Health as well as a generous equipment and supply allowance from the Biology Department of Case Western Reserve University to C.E.R. The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact.|
|Subject Keywords:||Drosophila melanogaster; alternate splicing; muscle; alternate polyadenylylation|
|Usage Policy:||No commercial reproduction, distribution, display or performance rights in this work are provided.|
|Deposited By:||Archive Administrator|
|Deposited On:||30 Oct 2006|
|Last Modified:||26 Dec 2012 09:14|
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