Jolicoeur, Paul and Baltimore, David (1975) Effect of the Fv-1 locus on the titration of murine leukemia viruses. Journal of Virology, 16 (6). pp. 1593-1598. ISSN 0022-538X http://resolver.caltech.edu/CaltechAUTHORS:JOLjvir75
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Titration of N- and B-tropic murine leukemia viruses on sensitive and resistant cell lines has been studied by direct XC plaque assay and infective center assay. The titration of cloned B-tropic virus by infective center assay on BALB/3T3 (Fv-1b/b) and NIH/3T3 (Fv-1n/n) cells gave one-hit patterns, with 100-fold less infected NIH/3T3 cells than BALB/3T3 cells. The titration of B-tropic virus on DBA/2 cells (Fv-1n/n) was also a one-hit. The titration of a one-hit curve, and there were about 100-fold less infected BALB/3T3 cells than NIH/3T3 cells. Comparable results were obtained by titrating the cloned N-tropic virus on congenic SIM (Fv-1n/n) and SIM.R (Fv-1b/b) cells or the Gross N-tropic virus on BALB/3T3 cells. Therefore, our data indicate that the multiple-hit phenomenon described previously may not be an essential part of the Fv-1 gene restriction.
|Additional Information:||Copyright © 1975 by the American Society for Microbiology. Received for publication 31 July 1975 We are grateful to T. Pincus, P. O'Donnell, and A. Axelrad for communicating their results before publication. We thank Nancy Hopkins for stimulating discussions and Anne Cotellesa for her excellent technical assistance. This work was supported by grant VC-4F from the American Cancer Society and Public Health Service grant CA-14051 from the National Cancer Institute. P.J. is a Fellow of the Medical Research Council ofCanada. D.B. is a Research Professor of the American Cancer Society.|
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|Deposited On:||01 Nov 2006|
|Last Modified:||26 Dec 2012 09:15|
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