Wagenaar, Daniel A. and Pine, Jerome and Potter, Steve M. (2006) Searching for plasticity in dissociated cortical cultures on multi-electrode arrays. Journal of Negative Results in BioMedicine, 5 (16). ISSN 1477-5751 http://resolver.caltech.edu/CaltechAUTHORS:WAGjnrb06
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We attempted to induce functional plasticity in dense cultures of cortical cells using stimulation through extracellular electrodes embedded in the culture dish substrate (multi-electrode arrays, or MEAs). We looked for plasticity expressed in changes in spontaneous burst patterns, and in array-wide response patterns to electrical stimuli, following several induction protocols related to those used in the literature, as well as some novel ones. Experiments were performed with spontaneous culture-wide bursting suppressed by either distributed electrical stimulation or by elevated extracellular magnesium concentrations as well as with spontaneous bursting untreated. Changes concomitant with induction were no larger in magnitude than changes that occurred spontaneously, except in one novel protocol in which spontaneous bursts were quieted using distributed electrical stimulation.
|Additional Information:||© 2006 Wagenaar et al., licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Submission date 2 June 2006. Acceptance date 26 October 2006. Publication date 26 October 2006 We thank our lab technician, Sheri McKinney. This work was partially supported by NINDS grants NS38628 (to SMP) and NS44134 (to JP), by NIBIB grant EB00786 (to SMP), by the Whitaker Foundation, and the Center for Behavioral Neuroscience. Competing interests: The authors declare that they have no competing interests. Authors’ contributions: DAW collected all data, performed the analysis, and prepared text and figures for the manuscript. JP and SMP contributed to the design of the study and to the preparation of the manuscript.|
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|Deposited On:||01 Nov 2006|
|Last Modified:||26 Dec 2012 09:15|
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