CaltechAUTHORS
  A Caltech Library Service

Circular Deoxyribonucleic Acid from Shigella dysenteriae Y6R

Rush, Mark G. and Gordon, C. N. and Warner, Robert C. (1969) Circular Deoxyribonucleic Acid from Shigella dysenteriae Y6R. Journal of Bacteriology, 100 (2). pp. 803-808. ISSN 0021-9193. http://resolver.caltech.edu/CaltechAUTHORS:RUSjbact69

[img]
Preview
PDF
See Usage Policy.

2170Kb

Use this Persistent URL to link to this item: http://resolver.caltech.edu/CaltechAUTHORS:RUSjbact69

Abstract

Circular deoxyribonucleic acid was isolated from Shigella dysenteriae Y6R and was found to consist of six species having molecular weights of 10^6, 1.3 x 10^6, 2.6 x 10^6, 3.8 x 10^6, 20 x 10^6, and 24 x 10^6 daltons. These size classes were partially resolved by sucrose density gradient centrifugation. The minicircles (10^6 and 1.3 x 10^6) were found to have a buoyant density in CsCl of 1.710 g/ml. The 3.8 x 10^6 dalton class had a density of 1.707 g/ml. The two largest species had a density of 1.702 g/ml. Two other strains, S. sonnei II and S. dysenteriae 60, also contained circular deoxyribonucleic acid.


Item Type:Article
Additional Information:Copyright © 1969 American Society for Microbiology. Received for publication 18 August 1969 This research was supported by Public Health Service grant GM 06967 from the National Institute of General Medical Sciences, by a grant for postdoctoral research from Merck and Co. (M.G.R.), by Public Health Service career award K3 GM 14899 (R.C.W.), and by a predoctoral fellowship from Public Health Service training grant 5 Ti GM 1234 (C.N.G.) The expert assistance of Dymetria Rush is gratefully acknowledged. ADDENDUM IN PROOF: Circular DNA has also been isolated from Shigella by H.S. Jansz, J. Zandberg, J.H. vander Pol, and E.F.J. van Bruggen (Eur. J. Biochem. 9:156, 1969).
Record Number:CaltechAUTHORS:RUSjbact69
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:RUSjbact69
Alternative URL:http://jb.asm.org/cgi/content/abstract/100/2/803
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:5795
Collection:CaltechAUTHORS
Deposited By: Archive Administrator
Deposited On:02 Nov 2006
Last Modified:26 Dec 2012 09:15

Repository Staff Only: item control page