Rush, Mark G. and Gordon, C. N. and Novick, Richard P. and Warner, Robert C. (1969) Penicillinase Plasmid DNA from Staphylococcus aureus. Proceedings of the National Academy of Sciences of the United States of America, 63 (4). pp. 1304-1310. ISSN 0027-8424. http://resolver.caltech.edu/CaltechAUTHORS:RUSpnas69
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A penicillinase plasmid from Staphylococcus aureus and three of its derivatives, all previously identified as extrachromosomal genetic elements, have been isolated in high yield as circular duplex DNA molecules. The wild-type plasmid was found by contour-length measurements of electron micrographs to have a molecular weight of 18.6 x 10^6 daltons. Two plasmids with deletions encompassing six and eight of the eleven known plasmid cistrons had molecular weights of 16.4 x 10^6 and 15.3 x 10^6 daltons, respectively. This information was used to establish approximate physical distances for the genetic map. A high-frequency transducing element also derived from the plasmid had a molecular weight of approximately 24 x 10^6 daltons. Although each plasmid preparation appeared homogeneous by ultracentrifugal analysis, electron micrographs always revealed the presence of a low percentage of complex oligomeric forms, particularly circular and catenated dimers.
|Additional Information:||Copyright © 1969 by the National Academy of Sciences Communicated by Severo Ochoa, June 13, 1969 We are grateful to Dymetria Rush for expert technical assistance. Aided by grants from the National Institutes of Health, U.S. Public Health Service, GM 06967 (R.C.W.) and GM 14372 (R.P.N.). [M.G.R. was a] Postdoctoral fellow supported by a grant from Merck and Co. [C.N.G. was a] Predoctoral trainee, U.S. Public Health Service training grant 5 TI GM 1234. [R.C.W. was a] Career awardee, U.S. Public Health Service, K3 GM 14899.|
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|Deposited On:||02 Nov 2006|
|Last Modified:||14 Nov 2014 19:19|
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