Haas, Martin and Altman, Amnon and Rothenberg, Ellen and Bogart, Mark H. and Jones, O. W. (1984) Mechanism of T-Cell Lymphomagenesis: Transformation of Growth-Factor-Dependent T-Lymphoblastoma Cells to Growth-Factor-Independent T-Lymphoma Cells. Proceedings of the National Academy of Sciences of the United States of America, 81 (6). pp. 1742-1746. ISSN 0027-8424 http://resolver.caltech.edu/CaltechAUTHORS:HAApnas84
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In a previous paper we described the induction by x-irradiation or radiation-induced leukemia virus-in-oculation of two classes of lymphoid T-cell neoplasms: The first class, designated T-cell lymphoblastoma (TCLB), consists of growth-factor-dependent eudiploid cells that home to the spleen and give rise to splenic tumors on injection into syngeneic mice; the second class, designated T-cell lymphoma (TCL), consists of growth-factor-independent aneuploid or pseudodiploid cells that give rise to local tumors at the site of subcutaneous injection. This paper describes the generation of a family of growth-factor-independent aneuploid or pseudodiploid TCL cells after the injection into the thymus of growth-factor-dependent diploid TCLB cells. In contrast to the donor TCLB cells, the resulting TCL cells could be cloned in semisolid medium, produced local tumors at the site of subcutaneous injection, and proliferated in a growth-factor-independent fashion in vitro. The induced growth-factor-independent TCL cells were chromosomally and phenotypically unstable and continued to evolve both in vivo and in vitro. After propagation in the thymus, the cells often showed stable translocations in addition to the evolving aneuploidy. We propose that the chromosome abnormalities induced during the proliferation of growth-factor-dependent TCLB cells in the thymus constitute a general mechanism by which neoplastic cells progress from growth-factor dependency to independency.
|Additional Information:||Copyright © 1984 by the National Academy of Sciences Communicated by Robert W. Holley, November 4, 1983 We thank Dr. Marguerite Vogt for invaluable advice and critical reading of the manuscript and members of the Molecular Biology and Virology Laboratory, The Salk Institute, for support. This work was supported by Grant CA 34151 from the National Cancer Institute, U.S. Public Health Service, and by Specialized Cancer Center Core Grant 5P30CA23100. The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact.|
|Subject Keywords:||cell propagation in thymic microenvironment; tumor progression; chromosomal abnormalities|
|Usage Policy:||No commercial reproduction, distribution, display or performance rights in this work are provided.|
|Deposited By:||Archive Administrator|
|Deposited On:||02 Nov 2006|
|Last Modified:||26 Dec 2012 09:15|
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