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DNA of Bacteriophage PM2: A Closed Circular Double-Stranded Molecule

Espejo, Romilio T. and Canelo, Eliana S. and Sinsheimer, Robert L. (1969) DNA of Bacteriophage PM2: A Closed Circular Double-Stranded Molecule. Proceedings of the National Academy of Sciences of the United States of America, 63 (4). pp. 1164-1168. ISSN 0027-8424. http://resolver.caltech.edu/CaltechAUTHORS:ESPpnas69

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Abstract

The DNA molecules isolated from mature bacteriophage PM2 are closed double-stranded rings of a molecular weight of six million. Direct evidence for the circularity and supercoiling of PM2 DNA was obtained by electron microscopy. Other properties, such as sedimentation behavior at neutral and alkaline pH, unusually high buoyant density in alkaline CsCl, and stability upon heating, are consistent with this structure. Only the four common nucleotides are found after hydrolysis and chromatographic analysis of PM2 DNA.


Item Type:Article
Additional Information:Copyright © 1969 by the National Academy of Sciences Communicated May 26, 1969 This research was supported in part by grant GM 13554 from the U.S. Public Health Service. M. lysodeikticus DNA and crab dAT DNA were generous gifts from Dr. J. Vinograd. We should like to acknowledge the assistance of Mr. Patrick Koen in performing the electron microscopy.
Record Number:CaltechAUTHORS:ESPpnas69
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:ESPpnas69
Alternative URL:http://www.pnas.org/cgi/content/abstract/63/4/1164
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:5813
Collection:CaltechAUTHORS
Deposited By: Archive Administrator
Deposited On:02 Nov 2006
Last Modified:26 Dec 2012 09:15

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