Interaction of Nucleosides and Related Compounds with Nucleic Acids as Indicated by the Change of Helix-Coil Transition Temperature

T'so, Paul O. P. and Helmkamp, G. K. and Sander, C. (1962) Interaction of Nucleosides and Related Compounds with Nucleic Acids as Indicated by the Change of Helix-Coil Transition Temperature. Proceedings of the National Academy of Sciences of the United States of America, 48 (4). pp. 686-698. ISSN 0027-8424 http://resolver.caltech.edu/CaltechAUTHORS:TSOpnas62

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Abstract

A series of compounds has been tested for effectiveness in lowering the melting temperature of poly A and of thymus DNA. The order of increasing activity was found to be: adonitol, methyl riboside (both negligible) < cyclohexanol < phenol, pyrimidine, uridine < cytidine, thymidine < purine, adenosine, inosine, deoxyguanosine < caffeine, coumarin, 2,6-dichloro-7-methylpurine. Urea was ineffective with poly A and only slightly effective with DNA. At a concentration of 0.3 M, purine lowered the Tm of DNA about 9°.

Item Type:	Article
Additional Information:	Copyright © 1962 by the National Academy of Sciences Communicated by James Bonner, February 27, 1962 We are grateful to Prof. James Bonner for his support in this work and to Prof. N. Davidson and Dr. J. Vinograd for their suggestions concerning the manuscript. The research was supported in part by grants no. RG-3977, no. RG-5143, and no. RG-8185(C1), National Institutes of Health.
Record Number:	CaltechAUTHORS:TSOpnas62
Persistent URL:	http://resolver.caltech.edu/CaltechAUTHORS:TSOpnas62
Alternative URL:	http://www.pnas.org/content/vol48/issue4/
Usage Policy:	No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:	5854
Collection:	CaltechAUTHORS
Deposited By:	Archive Administrator
Deposited On:	06 Nov 2006
Last Modified:	26 Dec 2012 09:15

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