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Genetically Encoded Spy Peptide Fusion System to Detect Plasma Membrane-Localized Proteins In Vivo

Bedbrook, Claire N. and Kato, Mihoko and Kumar, Sripriya Ravindra and Lakshmanan, Anupama and Nath, Ravi D. and Sun, Fei and Sternberg, Paul W. and Arnold, Frances H. and Gradinaru, Viviana (2015) Genetically Encoded Spy Peptide Fusion System to Detect Plasma Membrane-Localized Proteins In Vivo. Chemistry and Biology, 22 (8). pp. 1108-1121. ISSN 1074-5521. PMCID PMC4546540. http://resolver.caltech.edu/CaltechAUTHORS:20150728-090618874

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Abstract

Membrane proteins are the main gatekeepers of cellular state, especially in neurons, serving either to maintain homeostasis or instruct response to synaptic input or other external signals. Visualization of membrane protein localization and trafficking in live cells facilitates understanding the molecular basis of cellular dynamics. We describe here a method for specifically labeling the plasma membrane-localized fraction of heterologous membrane protein expression using channelrhodopsins as a case study. We show that the genetically encoded, covalent binding SpyTag and SpyCatcher pair from the Streptococcus pyogenes fibronectin-binding protein FbaB can selectively label membrane-localized proteins in living cells in culture and in vivo in Caenorhabditis elegans. The SpyTag/SpyCatcher covalent labeling method is highly specific, modular, and stable in living cells. We have used the binding pair to develop a channelrhodopsin membrane localization assay that is amenable to high-throughput screening for opsin discovery and engineering.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1016/j.chembiol.2015.06.020DOIArticle
http://www.sciencedirect.com/science/article/pii/S107455211500246XPublisherArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4546540/PubMed CentralArticle
ORCID:
AuthorORCID
Lakshmanan, Anupama0000-0002-6702-837X
Sternberg, Paul W.0000-0002-7699-0173
Arnold, Frances H.0000-0002-4027-364X
Gradinaru, Viviana0000-0001-5868-348X
Additional Information:© 2015 Elsevier Ltd. Received: February 10, 2015. Revised: May 19, 2015. Accepted: June 15, 2015. Published: July 23, 2015. We thank the Gradinaru and Arnold labs for helpful discussions, and Han Wang of the Sternberg lab for assistance with behavioral assays. We also thank Sheri McKinney for assistance with cultured neurons, and Dr. John Bedbrook for critical reading of the manuscript. This work was funded by the NIH/NINDS New Innovator (NIH IDP20D017782-01), the Beckman Institute for Optogenetics and CLARITY, the Gordon and Betty Moore Foundation through Grant GBMF2809 to the Caltech Programmable Molecular Technology Initiative (to V.G. and F.H.A.); by NIH 1R21MH103824-01 (to V.G. and F.H.A.); and by the Institute for Collaborative Biotechnologies through grant W911NF-09-0001 from the U.S. Army Research Office (to F.H.A.). The content of the information does not necessarily reflect the position or the policy of the Government, and no official endorsement should be inferred. P.W.S. is an investigator with the HHMI, which supported this research. C.N.B. acknowledges support from the NIMH of the NIH for the NRSA fellowship under Award Number F31MH102913.
Funders:
Funding AgencyGrant Number
NIHIDP20D017782-01
Caltech Beckman InstituteUNSPECIFIED
Gordon and Betty Moore FoundationGBMF2809
NIH1R21MH103824-01
Army Research Office (ARO)W911NF-09-0001
Howard Hughes Medical Institute (HHMI)UNSPECIFIED
NIH Predoctoral FellowshipF31MH102913
National Institute of Mental Health (NIMH)UNSPECIFIED
PubMed Central ID:PMC4546540
Record Number:CaltechAUTHORS:20150728-090618874
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20150728-090618874
Official Citation:Claire N. Bedbrook, Mihoko Kato, Sripriya Ravindra Kumar, Anupama Lakshmanan, Ravi D. Nath, Fei Sun, Paul W. Sternberg, Frances H. Arnold, Viviana Gradinaru, Genetically Encoded Spy Peptide Fusion System to Detect Plasma Membrane-Localized Proteins In Vivo, Chemistry & Biology, Volume 22, Issue 8, 20 August 2015, Pages 1108-1121, ISSN 1074-5521, http://dx.doi.org/10.1016/j.chembiol.2015.06.020. (http://www.sciencedirect.com/science/article/pii/S107455211500246X)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:59026
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:28 Jul 2015 16:27
Last Modified:08 Sep 2017 22:52

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