Levy, David E. and McKinnon, Randall D. and Brolaski, Mark N. and Gautsch, James W. and Wilson, Michael C. (1987) The 3' long terminal repeat of a transcribed yet defective endogenous retroviral sequence is a competent promoter of transcription. Journal of Virology, 61 (4). pp. 1261-1265. ISSN 0022-538X http://resolver.caltech.edu/CaltechAUTHORS:LEVjvir87
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Although actively transcribed and present as multiple genomic copies, a distinct class of endogenous murine leukemia virus-related sequence does not give rise to infectious virus. Since the long terminal repeat at the 3' terminus provides the transcriptional start site after reintegration, we determined the structure and potential promoter activity of that sequence obtained from cDNA of endogenous retroviral transcripts. These studies demonstrate that the distinctive 3' long terminal repeat sequence of these transcripts could serve as an effective promoter of transcription and, therefore, may not be the primary defect in the infectious cycle of retroviral replication but may result in the propagation of these endogenous retroviral sequences in the genome as retrotransposons.
|Additional Information:||Copyright © 1987 by the American Society for Microbiology. Received 28 August 1986/Accepted 16 December 1986 We thank H. Fan for his generous gift of the pUCCAT expression vector and M1CAT construct; J. Elder, P. Policastro, and G. Higgins for helpful comments during the preparation of the manuscript; and R. Evans and R. Ogata for many stimulating discussions concerning the maintenance and potential propagation of the retroviral sequences. We also acknowledge the patient secretarial help of M. Dietrich and P. Graber in the preparation of the manuscript. This work was supported in part by Public Health Service Predoctorial Training Grant GM-07616 to D.E.L. and grants CA-33730 (M.C.W.) and CA-35326 (J.W.G.) from the National Cancer Institute. R. McKinnon is a research fellow of the Canadian National Cancer Institute.|
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