A Caltech Library Service

An iron-regulated sortase anchors a class of surface protein during Staphylococcus aureus pathogenesis

Mazmanian, Sarkis K. and Ton-That, Hung and Su, Kenneth and Schneewind, Olaf (2002) An iron-regulated sortase anchors a class of surface protein during Staphylococcus aureus pathogenesis. Proceedings of the National Academy of Sciences of the United States of America, 99 (4). pp. 2293-2298. ISSN 0027-8424. PMCID PMC122358.

PDF - Published Version
See Usage Policy.


Use this Persistent URL to link to this item:


Sortase (SrtA), an enzyme that anchors surface proteins to the cell wall of Gram-positive bacteria, cleaves sorting signals at the LPXTG motif. We have identified a second sortase (SrtB) in the Gram-positive pathogen Staphylococcus aureus that is required for anchoring of a surface protein with a NPQTN motif. Purified SrtB cleaves NPQTN-bearing peptides in vitro, and a srtB mutant is defective in the persistence of animal infections. srtB is part of an iron-regulated locus called iron-responsive surface determinants (isd), which also contains a ferrichrome transporter and surface proteins with NPQTN and LPXTG motifs. Cell wall-anchored surface proteins and the isd locus seem involved in a novel mechanism of iron acquisition that is important for bacterial pathogenesis.

Item Type:Article
Related URLs:
URLURL TypeDescription CentralArticle
Mazmanian, Sarkis K.0000-0003-2713-1513
Additional Information:Copyright © 2002 by the National Academy of Sciences Edited by Christopher T. Walsh, Harvard Medical School, Boston, MA, and approved December 13, 2001 (received for review October 3, 2001). Published online before print February 5, 2002, 10.1073/pnas.032523999 We thank Simon Foster (University of Sheffield) for materials. S.K.M. acknowledges support from the Predoctoral Training Program in Genetics (T32GM07104) at the University of California, Los Angeles. Work in the O.S. laboratory is supported by National Institutes of Health-National Institute of Allergy and Infectious Diseases, Infectious Disease Branch Grant AI33987. This paper was submitted directly (Track II) to the PNAS office.
Funding AgencyGrant Number
NIH Predoctoral Training ProgramT32GM07104
National Institute of Allergy and Infectious DiseasesAI33987
PubMed Central ID:PMC122358
Record Number:CaltechAUTHORS:MAZpnas02
Persistent URL:
Alternative URL:
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:5990
Deposited By: Archive Administrator
Deposited On:10 Nov 2006
Last Modified:15 Oct 2015 22:36

Repository Staff Only: item control page