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Q-band electron nuclear double resonance (ENDOR) and X-band EPR of the sulfobetaine 12 heat-treated cytochrome c oxidase complex

Musser, Siegfried M. and Fann, Yang-Cheng and Gurbiel, Ryszard J. and Hoffman, Brian M. and Chan, Sunney I. (1997) Q-band electron nuclear double resonance (ENDOR) and X-band EPR of the sulfobetaine 12 heat-treated cytochrome c oxidase complex. Journal of Biological Chemistry, 272 (1). pp. 203-209. ISSN 0021-9258. http://resolver.caltech.edu/CaltechAUTHORS:MUSjbc97

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Abstract

Heat treatment of the bovine cytochrome c oxidase complex in the zwitterionic detergent sulfobetaine 12 (SB-12) results in loss of subunit III and the appearance of a type II copper center as characterized by electron paramagnetic resonance (EPR) spectroscopy. Previous authors (Nilsson, T., Copeland, R. A., Smith, P. A., and Chan, S. I. (1988) Biochemistry 27, 8254-8260) have interpreted this type II copper center as a modified version of the CuA site. By using electron nuclear double resonance spectroscopy, it is found that the CuA proton and nitrogen resonances remain present in the SB-12 heat-treated enzyme and that three new nitrogen resonances appear having hyperfine coupling constants consistent with histidine ligation. These hyperfine coupling constants correlate well with those recently found for the CuB histidines from the cytochrome aa3-600 quinol oxidase from Bacillus subtilis (Fann, Y. C., Ahmed, I., Blackburn, N. J., Boswell, J. S., Verkhovskaya, M. L., Hoffman, B. M., and Wikström, M. (1995) Biochemistry 34, 10245-10255). In addition, the total EPR-detectable copper concentration per enzyme molecule approximately doubles upon SB-12 heat treatment. Finally, the observed type II copper EPR spectrum is virtually indistinguishable from the EPR spectrum of CuB of the as-isolated cytochrome bo3 complex from Escherichia coli. These data indicate that the type II copper species that appears results from a breaking of the strong antiferromagnetic coupling of the heme a3-CuB binuclear center.


Item Type:Article
Additional Information:©1997 by The American Society for Biochemistry and Molecular Biology, Inc. Received for publication, July 16, 1996, and in revised form, October 10, 1996. Special thanks to Mårten Wikström for sharing manuscripts before publication and to Michael Stowell for help in the isolation of the cytochrome bo3 complex. This work was supported by National Institutes of Health Grants HL13531 (to B.M.H.) and GM22432 (to S.I.C.) from the United States Public Health Service. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked “advertisement” in accordance with 18 U.S.C. Section 1734 solely to indicate this fact. [S.M.M. was the] Recipient of a National Research Service Predoctoral Award.
Subject Keywords:THERMUS-THERMOPHILUS; COPPER CENTER; SPECTROSCOPIC CHARACTERIZATION; PARACOCCUS-DENITRIFICANS; REDUCTASE CONTAINS; QUINOL OXIDASE; SITE; PROTEINS; SUBUNIT; DOMAIN
Record Number:CaltechAUTHORS:MUSjbc97
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:MUSjbc97
Alternative URL:http://www.jbc.org/cgi/content/abstract/272/1/203
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:6000
Collection:CaltechAUTHORS
Deposited By: Lindsay Cleary
Deposited On:13 Nov 2006
Last Modified:26 Dec 2012 09:16

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