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Dual function of Slit2 in repulsion and enhanced migration of trunk, but not vagal, neural crest cells

De Bellard, Maria Elena and Rao, Yi and Bronner-Fraser, Marianne (2003) Dual function of Slit2 in repulsion and enhanced migration of trunk, but not vagal, neural crest cells. Journal of Cell Biology, 162 (2). pp. 269-279. ISSN 0021-9525. http://resolver.caltech.edu/CaltechAUTHORS:BELjcb03

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Abstract

Neural crest precursors to the autonomic nervous system form different derivatives depending upon their axial level of origin; for example, vagal, but not trunk, neural crest cells form the enteric ganglia of the gut. Here, we show that Slit2 is expressed at the entrance of the gut, which is selectively invaded by vagal, but not trunk, neural crest. Accordingly, only trunk neural crest cells express Robo receptors. In vivo and in vitro experiments demonstrate that trunk, not vagal, crest cells avoid cells or cell membranes expressing Slit2, thereby contributing to the differential ability of neural crest populations to invade and innervate the gut. Conversely, exposure to soluble Slit2 significantly increases the distance traversed by trunk neural crest cells. These results suggest that Slit2 can act bifunctionally, both repulsing and stimulating the motility of trunk neural crest cells.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1083/jcb.200301041DOIArticle
ORCID:
AuthorORCID
Bronner-Fraser, Marianne0000-0003-4274-1862
Additional Information:©2003 The Rockefeller University Press. Submitted: 13 January 2003; revised: 11 June 2003; accepted: 16 June 2003. We thank Scott Fraser for helpful comments on the manuscript, Martin Garcia-Castro and Sara Ahlgren for help in analyzing the data, and Gustavo Gomez, Vivian Lee, and Joanne Tan-Cabugao for technical support. This work was supported in part by a postdoctoral fellowship to M.E. De Bellard from the National Multiple Sclerosis Society (FA 1383-A-1) and by a United States Public Health Service grant (HD-15527) to M. Bronner-Fraser. The supplemental material (Fig. S1 and Videos 1 and 2) is available at http://www.jcb.org/cgi/content/full/jcb.200301041/DC1. The movies show neural crest cells moving from three different experiments combined together for control and Slit2-exposed neural crest cells. The lapsed time is ~2.5 h. Videos 1 (4.2 MB) and 2 (8.3 MB) -- Slit2 enhances neural crest cell migration. Trunk neural tubes were cultured overnight on fibronectin. Cells were vitally labeled with CalceinAM (Molecular Probes) for confocal visualization after media was changed to one conditioned by control (HEK293 cells) (Video 2) or Slit2-secreting cells (Video 1) 1 h before video microscopy in a 410 LSM confocal for 3 h. Movies show three different experiments combined together for control and Slit2-exposed neural crest cells.
Funders:
Funding AgencyGrant Number
National Multiple Sclerosis SocietyFA 1383-A-1
U.S. Public Health Service (USPHS)HD-15527
Subject Keywords:Slit2; neural crest; vagal; chemorepellent; gut
Record Number:CaltechAUTHORS:BELjcb03
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:BELjcb03
Alternative URL:http://dx.doi.org/10.1083/jcb.200301041
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:6019
Collection:CaltechAUTHORS
Deposited By: Lindsay Cleary
Deposited On:14 Nov 2006
Last Modified:02 Jan 2016 07:41

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