Use of dyes to increase phase contrast for biological holographic microscopy
Abstract
Holographic microscopy is an emerging biological technique that provides amplitude and quantitative phase imaging, though the contrast provided by many cell types and organelles is low, and until now no dyes were known that increased contrast. Here we show that the metallocorrole Ga(tpfc)(SO_3H)_2, which has a strong Soret band absorption, increases contrast in both amplitude and phase and facilitates tracking of Escherichia coli with minimal toxicity. The change in phase contrast may be calculated from the dye-absorbance spectrum using the Kramers–Kronig relations, and represents a general principle that may be applied to any dye or cell type. This enables the use of holographic microscopy for all applications in which specific labeling is desired.
Additional Information
© 2015 Optical Society of America. Received 16 June 2015; revised 22 July 2015; accepted 30 July 2015; posted 4 August 2015 (Doc. ID 243160); published 28 August 2015. Funding. Gordon and Betty Moore Foundation (4037, 4038). Acknowledgment. We thank J. Kent Wallace for useful discussions and John Termini for the gift of the dye.Attached Files
Published - ol-40-17-4114.pdf
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Additional details
- Eprint ID
- 60689
- Resolver ID
- CaltechAUTHORS:20151001-110324966
- 4037
- Gordon and Betty Moore Foundation
- 4038
- Gordon and Betty Moore Foundation
- Created
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2015-10-01Created from EPrint's datestamp field
- Updated
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2021-11-10Created from EPrint's last_modified field