Hwang, Karl J. and Mauk, Marcia R. (1977) Fate of Lipid Vesicles in vivo: A Gamma-Ray Perturbed Angular Correlation Study. Proceedings of the National Academy of Sciences of the United States of America, 74 (11). pp. 4991-4995. ISSN 0027-8424 http://resolver.caltech.edu/CaltechAUTHORS:HWApnas77
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The structural integrity of unilamellar vesicles has been studied in vitro and in vivo by use of gamma-ray perturbed angular correlation techniques. These studies utilize 111In3+ weakly bound to the chelator nitrilotriacetic acid as a probe to monitor the percentage of intact vesicles. When complexes of 111In3+ and nitrilotriacetic acid is encapsulated in vesicles, 111In3+ exhibits a fast tumbling rate. Upon alteration of the membrane, 111In3+ is released from the liposomes and becomes bound to macromolecules, consequently exhibiting a decrease in 111In3+ tumbling rate. The in vitro experiments show that the present technique is capable of determining quantitatively the percentage of material released from the vesicles upon the addition of serum or Triton X-100 to vesicles. The percentage of vesicles remaining intact in vivo can also be monitored continuously by the present technique. In mice, the half-life of dipalmitoyl phosphatidylcholine-cholesterol vesicles after intraperitoneal injection was estimated to be 10-13 hr. The present study suggests that the vesicles remain intact in various tissues for extended periods, thereby allowing a slow release of the encapsulated material at those sites.
|Additional Information:||Copyright © 1977 by the National Academy of Sciences Communicated by John D. Baldeschwieler, August 12, 1977 We gratefully acknowledge the encouragement and support of Prof. John D. Baldeschwieler. We also thank our colleagues in this laboratory, particularly Dr. R.C. Gamble for his stimulating discussions and A.Y. Hwang for her helpful technical assistance. This investigation was supported by the National Institutes of Health Grant GM-21111-03 and National Science Foundation Grant GP-38855X-3. This is contribution 5596 from the Arthur Amos Noyes Laboratory of Chemical Physics. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U. S. C. §1734 solely to indicate this fact.|
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|Deposited On:||17 Nov 2006|
|Last Modified:||26 Dec 2012 09:17|
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