Benzer, Seymour (1955) Fine Structure of a Genetic Region in Bacteriophage. Proceedings of the National Academy of Sciences of the United States of America, 41 (6). pp. 344-354. ISSN 0027-8424. http://resolver.caltech.edu/CaltechAUTHORS:BENpnas55
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This paper describes a functionally related region in the genetic material of a bacteriophage that is finely subdivisible by mutation and by genetic recombination. The group of mutants resembles similar cases which have been observed in many organisms, usually designated as "pseudo-alleles." (See reviews by Lewis  and Pontecorvo .) Such cases are of special interest for their bearing on the structure and function of genetic determinants. The phenomenon of genetic recombination provides a powerful tool for separating mutations and discerning their positions along a chromosome. When it comes to very closely neighboring mutations, a difficulty arises, since the closer two mutations lie to one another, the smaller is the probability that recombination between them will occur. Therefore, failure to observe recombinant types among a finite number of progeny ordinarily does not justify the conclusion that the two mutations are inseparable but can only place an upper limit on the linkage distance between them. A high degree of resolution requires the examination of very many progeny. This can best be achieved if there is available a selective feature for the detection of small proportions of recombinants. Some preliminary results are here presented of a program designed to extend genetic studies to the molecular (nucleotide) level.
|Additional Information:||Copyright © 1955 by the National Academy of Sciences Communicated by M. Delbrück, April 6, 1955 I am much indebted to A. D. Hershey and A. H. Doermann for stocks of their genetically mapped mutants, to Sydney Brenner and David Krieg for stimulating discussion, and to Max Delbrück for his invaluable moderating influence. Supported by a grant-in-aid from the American Cancer Society upon recommendation of the Committee on Growth of the National Research Council.|
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|Deposited On:||28 Nov 2006|
|Last Modified:||14 Nov 2014 19:19|
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