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The absence of α-aminoadipic acid in cholera Vibrio

Windsor, Emanuel (1950) The absence of α-aminoadipic acid in cholera Vibrio. Journal of Biological Chemistry, 182 (2). pp. 699-700. ISSN 0021-9258. http://resolver.caltech.edu/CaltechAUTHORS:WINjbc50

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Abstract

Blass and Macheboeuf reported that they had isolated α-aminoadipic acid from cholera Vibrio (1). On repeating the experiment later, they tested the purity of the isolated product by means of paper chromatograms developed with phenol and retracted their earlier claim (2). However, Borsook et al. found that α-aminoadipic acid and glutamic acid gave the same spot on two-dimensional paper chromatograms developed with phenol and δ-collidine (3). The retraction of Blass and Macheboeuf was therefore based on a method which does not differentiate between the two dicarboxylic amino acids. By using the starch chromatographic method of Stein and Moore (4), Borsook et al. were able to separate α-aminoadipic acid from other amino acids, including glutamic acid (3). In this present study an attempt is made by the same technique to determine whether α-aminoadipic acid is present in the cholera Vibrio.


Item Type:Article
Additional Information:Copyright © 1950 by the American Society for Biochemistry and Molecular Biology. (Received for publication, October 3, 1949) The radioactive α-aminoadipic acid was obtained through the courtesy of Dr. Peter H. Lowy. The C14 used in this investigation was supplied by the Monsanto Chemical Company, Clinton Laboratories, and obtained on allocation from the United States Atomic Energy Commission.
Record Number:CaltechAUTHORS:WINjbc50
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:WINjbc50
Alternative URL:http://www.jbc.org/content/vol182/issue2/index.shtml
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ID Code:6205
Collection:CaltechAUTHORS
Deposited By: Archive Administrator
Deposited On:28 Nov 2006
Last Modified:26 Dec 2012 09:18

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