Weisblum, Bernard and Benzer, Seymour and Holley, Robert W. (1962) A Physical Basis for Degeneracy in the Amino Acid Code. Proceedings of the National Academy of Sciences of the United States of America, 48 (8). pp. 1449-1454. ISSN 0027-8424 http://resolver.caltech.edu/CaltechAUTHORS:WEIpnas62
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If two or more nucleotide sequences specify the same amino acid, the code is said to be degenerate for that amino acid. Evidence that such degeneracy exists in E. coli comes from experiments on the stimulation of ribosomal incorporation of amino acids by synthetic polyribonucleotides. For instance, Martin et al.  and Speyer et al.  found that either poly UC or poly UG stimulates incorporation of leucine, so that at least two different combinations code for the same amino acid. Since the specificity of coding seems to reside in sRNA molecules functioning as adaptors [3,4], such degeneracy might be due to the existence of two sRNA acceptors for leucine, each having different coding properties. Two peaks of leucine-acceptor activity were demonstrated in yeast sRNA by Doctor et al.  using countercurrent distribution. Berg and Lagerkvist  have shown that E. coli sRNA also contains two leucine acceptors with different acceptor-terminal nucleotide sequences. In this paper, two leucine acceptors in E. coli, separated by countercurrent distribution, are shown to have different coding properties. One responds preferentially to poly UC, the other to poly UG.
|Additional Information:||Copyright © 1962 by the National Academy of Sciences Communicated June 25, 1962 This research was supported by grants from the National Science Foundation and the National Institutes of Health. One of us (B.W.) is the holder of a National Institutes of Health Postdoctoral Fellowship.|
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|Deposited On:||29 Nov 2006|
|Last Modified:||26 Dec 2012 09:18|
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