Weisman, Lois S. and Emr, Scott D. and Wickner, William T. (1990) Mutants of Saccharomyces cerevisiae that Block Intervacuole Vesicular Traffic and Vacuole Division and Segregation. Proceedings of the National Academy of Sciences of the United States of America, 87 (3). pp. 1076-1080. ISSN 0027-8424 http://resolver.caltech.edu/CaltechAUTHORS:WEIpnas90
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Intervacuole vesicular exchange and the segregation of parental vacuole material into the bud are strikingly impaired in a temperature-sensitive yeast mutant, vac1-1. At the nonpermissive temperature, haploid vac1-1 cells show a pronounced delay in separation of mature buds from the mother cell and accumulate cells with multiple buds. At both the permissive and restrictive temperatures, daughter cells are produced that lack a detectable vacuole or contain a very small vacuole. In zygotes, vacuoles from a vac1-1 strain are defective as donors, or recipients, of the vesicles of intervacuole vesicular traffic. These defects are specific for the vacuole in that the segregation of nuclear DNA and of mitochondria into the bud appears normal. The isolation of the vac1-1 mutation is a first step in the genetic characterization of vacuole division and segregation.
|Additional Information:||Copyright © 1990 by National Academy of Sciences Communicated by Mary Jane Osborn, October 26, 1989 This work was supported by National Institutes of Health Grant GM38895 and by a fellowship to L.S.W. from Atherosclerosis Training Grant HL07386. The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact.|
|Subject Keywords:||lysosome; organelle inheritance; VAC1|
|Usage Policy:||No commercial reproduction, distribution, display or performance rights in this work are provided.|
|Deposited By:||Archive Administrator|
|Deposited On:||29 Nov 2006|
|Last Modified:||26 Dec 2012 09:18|
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