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A DNA-Protein Complex Involved in Bacteriophage phi X174 Particle Formation

Weisbeek, Peter J. and Sinsheimer, Robert L. (1974) A DNA-Protein Complex Involved in Bacteriophage phi X174 Particle Formation. Proceedings of the National Academy of Sciences of the United States of America, 71 (8). pp. 3054-3058. ISSN 0027-8424. PMCID PMC388619. http://resolver.caltech.edu/CaltechAUTHORS:WEIpnas74

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Abstract

A phi X-specific DNA-protein complex has been isolated from phi X-infected cells. This complex contains infective circular single-stranded DNA, the proteins of the genes F, G, H, and J in the same proportions as in the phage particle, and, in addition, the gene D-protein. The D-protein makes up 12-22% of the protein part of the complex. The sedimentation value of the complex is about 140 S. In vitro, the complex can be converted to the normal 114S phage particle (with a concomitant loss of its D-protein) or to an uninfective 70S particle and a small amount of free single-stranded DNA. The fast sedimenting particle is not associated with membranes.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC388619/PubMed CentralArticle
Additional Information:Copyright © 1974 by the National Academy of Sciences Contributed by Robert L. Sinsheimer, May 16, 1974 This research was supported by a NATO Science Fellowship given to P.J.W. by the Netherlands Organization for the Advancement of Pure Research (Z.W.O.) and by Grant GM 13554 from the United States Public Health Service.
Funders:
Funding AgencyGrant Number
North Atlantic Treaty Organization (NATO)UNSPECIFIED
Nederlandse Organisatie voor Wetenschappelijk Onderzoek (NWO)UNSPECIFIED
U.S. Public Health Service (USPHS)GM 13554
Subject Keywords:intermediates; assembly; gene D-protein
PubMed Central ID:PMC388619
Record Number:CaltechAUTHORS:WEIpnas74
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:WEIpnas74
Alternative URL:http://www.pnas.org/cgi/content/abstract/71/8/3054
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:6231
Collection:CaltechAUTHORS
Deposited By: Archive Administrator
Deposited On:29 Nov 2006
Last Modified:16 Dec 2015 21:12

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