Weisbeek, Peter J. and Sinsheimer, Robert L. (1974) A DNA-Protein Complex Involved in Bacteriophage phi X174 Particle Formation. Proceedings of the National Academy of Sciences of the United States of America, 71 (8). pp. 3054-3058. ISSN 0027-8424 http://resolver.caltech.edu/CaltechAUTHORS:WEIpnas74
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A phi X-specific DNA-protein complex has been isolated from phi X-infected cells. This complex contains infective circular single-stranded DNA, the proteins of the genes F, G, H, and J in the same proportions as in the phage particle, and, in addition, the gene D-protein. The D-protein makes up 12-22% of the protein part of the complex. The sedimentation value of the complex is about 140 S. In vitro, the complex can be converted to the normal 114S phage particle (with a concomitant loss of its D-protein) or to an uninfective 70S particle and a small amount of free single-stranded DNA. The fast sedimenting particle is not associated with membranes.
|Additional Information:||Copyright © 1974 by the National Academy of Sciences Contributed by Robert L. Sinsheimer, May 16, 1974 This research was supported by a NATO Science Fellowship given to P.J.W. by the Netherlands Organization for the Advancement of Pure Research (Z.W.O.) and by Grant GM 13554 from the United States Public Health Service.|
|Subject Keywords:||intermediates; assembly; gene D-protein|
|Usage Policy:||No commercial reproduction, distribution, display or performance rights in this work are provided.|
|Deposited By:||Archive Administrator|
|Deposited On:||29 Nov 2006|
|Last Modified:||26 Dec 2012 09:19|
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