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A DNA-Protein Complex Involved in Bacteriophage phi X174 Particle Formation

Weisbeek, Peter J. and Sinsheimer, Robert L. (1974) A DNA-Protein Complex Involved in Bacteriophage phi X174 Particle Formation. Proceedings of the National Academy of Sciences of the United States of America, 71 (8). pp. 3054-3058. ISSN 0027-8424. http://resolver.caltech.edu/CaltechAUTHORS:WEIpnas74

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Abstract

A phi X-specific DNA-protein complex has been isolated from phi X-infected cells. This complex contains infective circular single-stranded DNA, the proteins of the genes F, G, H, and J in the same proportions as in the phage particle, and, in addition, the gene D-protein. The D-protein makes up 12-22% of the protein part of the complex. The sedimentation value of the complex is about 140 S. In vitro, the complex can be converted to the normal 114S phage particle (with a concomitant loss of its D-protein) or to an uninfective 70S particle and a small amount of free single-stranded DNA. The fast sedimenting particle is not associated with membranes.


Item Type:Article
Additional Information:Copyright © 1974 by the National Academy of Sciences Contributed by Robert L. Sinsheimer, May 16, 1974 This research was supported by a NATO Science Fellowship given to P.J.W. by the Netherlands Organization for the Advancement of Pure Research (Z.W.O.) and by Grant GM 13554 from the United States Public Health Service.
Subject Keywords:intermediates; assembly; gene D-protein
Record Number:CaltechAUTHORS:WEIpnas74
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:WEIpnas74
Alternative URL:http://www.pnas.org/cgi/content/abstract/71/8/3054
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:6231
Collection:CaltechAUTHORS
Deposited By: Archive Administrator
Deposited On:29 Nov 2006
Last Modified:14 Nov 2014 19:19

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