Kim, Sunyoung and Ikeuchi, Kenji and Groopman, Jerome and Baltimore, David (1990) Factors affecting cellular tropism of human immunodeficiency virus. Journal of Virology, 64 (11). pp. 5600-5604. ISSN 0022-538X http://resolver.caltech.edu/CaltechAUTHORS:KIMjvir90
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To evaluate the basis of the slow growth of many human immunodeficiency virus strains in monocytes/macrophages, various stages of the virus life cycle have been studied for their possible contribution to viral tropism. Although we found that monocytic U937 cells had a higher percentage of CD4-positive cells than T-lymphoid H9 cells, the human immunodeficiency virus strain grew much less efficiently in the monocytic line. Viral tropism was primarily determined during the early stages of the virus cycle, that is, sometime between binding of the virus to the cell surface and reverse transcription of viral genomic RNA. Once the virus entered the host cell, reverse transcription, use of the long terminal repeat, RNA expression, and production of virus particles was about as efficient in monocytes as in T cells. Thus, during viral entry into the host cell cytoplasm there is a major limiting event that is particularly inefficient in U937 cells and possibly in all monocytes/macrophages.
|Additional Information:||Copyright © 1990 by the American Society for Microbiology. Received 29 March 1990/Accepted 10 August 1990 We thank Mary A. Harbison and Paula Cannon for reading the manuscript. S.K. is a fellow of the Jane Coffin Childs Memorial Fund for Medical Research. This work was supported in part by Pubic Health Service grants P01 HL43510 and U01 A126463 (to D.B.) and DAMO 17-87-C-7017, R01 Al 24475, HL42112, HL 33774, HL41374, and HL43510 (to J.G.) from the National Institutes of Health.|
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|Deposited On:||30 Nov 2006|
|Last Modified:||26 Dec 2012 09:19|
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