Kim, Sunyoung and Byrn, Randal and Groopman, Jerome and Baltimore, David (1989) Temporal aspects of DNA and RNA synthesis during human immunodeficiency virus infection: evidence for differential gene expression. Journal of Virology, 63 (9). pp. 3708-3713. ISSN 0022-538X http://resolver.caltech.edu/CaltechAUTHORS:KIMjvir89
See Usage Policy.
Use this Persistent URL to link to this item: http://resolver.caltech.edu/CaltechAUTHORS:KIMjvir89
The kinetics of retroviral DNA and RNA synthesis are parameters vital to understanding viral growth, especially for human immunodeficiency virus (HIV), which encodes several of its own regulatory genes. We have established a single-cycle growth condition for HIV in H9 cells, a human CD4+ lymphocyte line. The full-length viral linear DNA is first detectable by 4 h postinfection. During a one-step growth of HIV, amounts of viral DNA gradually increase until 8 to 12 h postinfection and then decrease. The copy number of unintegrated viral DNA is not extraordinarily high even at its peak. Most strikingly, there is a temporal program of RNA accumulation: the earliest RNA is greatly enriched in the 2-kilobase subgenomic mRNA species, while the level of 9.2-kilobase RNA which is both genomic RNA and mRNA remains low until after 24 h of infection. Virus production begins at about 24 h postinfection. Thus, viral DNA synthesis is as rapid as for other retroviruses, but viral RNA synthesis involves temporal alteration in the species that accumulate, presumably as a consequence of viral regulatory genes.
|Additional Information:||Copyright © 1989 by the American Society for Microbiology. Received 13 March 1989/Accepted 24 May 1989 We thank Harold Varmus and Mark Schlissel for critically reading the manuscript, Mark Feinberg for providing some plasmids used for the construction of pWI3, and Kenji Ikeuchi for help in preparation of primary T cells. S.K. is a fellow of the Jane Coffin Childs Memorial Fund for Medical Research. This work was supported in part by Public Health Service grants HL33774, HL41374, HL42112, A123627, and A124475 to R.B. and J.G. and GM39458 and A126463 to D.B. from the National Institutes of Health and by Defense Department contract DAMD 17-87-C-7017 to R.B. and J.G.|
|Usage Policy:||No commercial reproduction, distribution, display or performance rights in this work are provided.|
|Deposited By:||Archive Administrator|
|Deposited On:||30 Nov 2006|
|Last Modified:||26 Dec 2012 09:19|
Repository Staff Only: item control page