CaltechAUTHORS
  A Caltech Library Service

The accumulation of orotic acid by a pyrimidineless mutant of Neurospora

Mitchell, Herschel K. and Houlahan, Mary B. and Nyc, Joseph F. (1948) The accumulation of orotic acid by a pyrimidineless mutant of Neurospora. Journal of Biological Chemistry, 172 (2). pp. 525-529. ISSN 0021-9258. http://resolver.caltech.edu/CaltechAUTHORS:MITjbc48a

[img]
Preview
PDF
See Usage Policy.

275Kb

Use this Persistent URL to link to this item: http://resolver.caltech.edu/CaltechAUTHORS:MITjbc48a

Abstract

The discovery of orotic acid (1,2-carboxyuracil) in cow's milk by Biscaro and Belloni (1), followed by its identification and synthesis (2-4), led to a number of speculations as to its biological origin and significance (,3,5,6). A definite connection of orotic acid with the biosynthesis of nucleic acid pyrimidines is provided by the finding that orotic acid (7) as well as thymine (8,9) can supplement or replace the folic acid required by certain microorganisms. As suggested by Chattaway (7), it would appear that folic acid has a function in the biosynthesis of pyrimidines. Furthermore, this function is probably concerned in some step prior to the appearance of orotic acid in the biosynthetic series. More recently it was shown by Loring and Pierce (10) that orotic acid could be substituted for uracil in satisfying the growth requirements of some pyrimidineless mutants of the mold Neurospora. Investigations of orotic acid in this laboratory have led to a new method of synthesis of the compound (11) and to some suggestions concerning its relation to the biosynthesis of nucleic acids in Neurospora (12). The results of the present work are in accord with the previous suggestions and provide further evidence on the biological origin and function of orotic acid.


Item Type:Article
Additional Information:Copyright © 1948 by the American Society for Biochemistry and Molecular Biology. Received for publication, October 27, 1947 These investigations were supported by the Rockefeller Foundation and the Williams-Waterman Fund for the Combat of Dietary Diseases.
Record Number:CaltechAUTHORS:MITjbc48a
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:MITjbc48a
Alternative URL:http://www.jbc.org/content/vol172/issue2/index.shtml
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:6445
Collection:CaltechAUTHORS
Deposited By: Archive Administrator
Deposited On:09 Dec 2006
Last Modified:26 Dec 2012 09:21

Repository Staff Only: item control page