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Regulation of Sphingosine-1-phosphate Lyase Gene Expression by Members of the GATA Family of Transcription Factors

Oskouian, Babak and Mendel, Jane and Shocron, Ellyn and Lee, Michael A., Jr. and Fyrst, Henrik and Saba, Julie D. (2005) Regulation of Sphingosine-1-phosphate Lyase Gene Expression by Members of the GATA Family of Transcription Factors. Journal of Biological Chemistry, 280 (18). pp. 18403-18410. ISSN 0021-9258. http://resolver.caltech.edu/CaltechAUTHORS:OSKjbc05

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Abstract

Sphingosine-1-phosphate is a bioactive sphingolipid that regulates proliferation, differentiation, migration, and apoptosis. Sphingosine-1-phosphate is irreversibly degraded by the highly conserved enzyme sphingosine-1-phosphate lyase. Recent studies have suggested that sphingosine-1-phosphate lyase expression affects animal development and cell fate decisions. Despite its crucial role, mechanisms affecting expression of sphingosine-1-phosphate lyase remain poorly understood. In this study, regulation of sphingosine-1-phosphate lyase gene expression was investigated in Caenorhabditis elegans, where lyase expression is spatially restricted to cells of the developing and adult gut and is essential for normal development. Deletion analysis and generation of transgenic worms combined with fluorescence microscopy identified a 350-nucleotide sequence upstream of the ATG start site necessary for maximal lyase expression in adult worms. Site-specific mutagenesis of a GATA transcription factor-binding motif in the promoter led to loss of reporter expression. Knockdown of the gut-specific GATA transcription factor ELT-2 by RNA interference similarly led to loss of reporter expression. ELT-2 interacted with the GATA factor-binding motif in vitro and was also capable of driving expression of a Caenorhabditis elegans lyase promoter-{beta}-galactosidase reporter in a heterologous yeast system. These studies demonstrate that ELT-2 regulates sphingosine-1-phosphate lyase expression in vivo. Additionally, we demonstrate that the human sphingosine-1-phosphate lyase gene is regulated by a GATA transcription factor. Overexpression of GATA-4 led to both an increase in activity of a reporter gene as well as an increase in endogenous sphingosine-1-phosphate lyase protein.


Item Type:Article
Additional Information:Copyright © 2005 by the American Society for Biochemistry and Molecular Biology. Received for publication, September 22, 2004, and in revised form, February 9, 2005. Originally published In Press as doi:10.1074/jbc.M410928200 on February 25, 2005 We thank James D. McGhee for generosity with reagents, critical reading of the manuscript, and for many helpful discussions; Joel Rothman and Keith Strohmaier for the use of ELT-7 constructs; Paul Sternberg for helpful discussions, and Betsy Lathrop for expert administrative assistance. This work was supported by National Institutes of Health Grant 1R01CA77528 (to J. D. S.). The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
Record Number:CaltechAUTHORS:OSKjbc05
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:OSKjbc05
Alternative URL:http://dx.doi.org/10.1074/jbc.M410928200
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:6448
Collection:CaltechAUTHORS
Deposited By: Archive Administrator
Deposited On:09 Dec 2006
Last Modified:26 Dec 2012 09:21

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