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Restriction Fragment Length Polymorphism Linkage Map for Arabidopsis thaliana

Chang, Caren and Bowman, John L. and DeJohn, Arthur W. and Lander, Eric S. and Meyerowitz, Elliot M. (1988) Restriction Fragment Length Polymorphism Linkage Map for Arabidopsis thaliana. Proceedings of the National Academy of Sciences of the United States of America, 85 (18). pp. 6856-6860. ISSN 0027-8424. http://resolver.caltech.edu/CaltechAUTHORS:CHApnas88

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Abstract

We have constructed a restriction fragment length polymorphism linkage map for the nuclear genome of the flowering plant Arabidopsis thaliana. The map, containing 90 randomly distributed molecular markers, is physically very dense; >50% of the genome is within 1.9 centimorgans, or approx 270 kilobase pairs, of the mapped DNA fragments. The map was based on the meiotic segregation of markers in two different crosses. The restriction fragment length polymorphism linkage groups were integrated with the five classically mapped linkage groups by virtue of mapped mutations included in these crosses. Markers consist of both cloned Arabidopsis genes and random low-copy-number genomic DNA clones that are able to detect polymorphisms with the restriction enzymes EcoRI, Bgl II, and/or Xba I. These cloned markers can serve as starting points for chromosome walking, allowing for the isolation of Arabidopsis genes of known map location. The restriction fragment length polymorphism map also can associate clones of unknown gene function with mutant phenotypes, and vice versa.


Item Type:Article
Additional Information:Copyright © 1988 by The National Academy of Sciences. Communicated by E. B. Lewis, June 8, 1988. We are grateful to the following people for their valuable contributions to this work: Robert Pruitt for constructing the triply marked line and performing the cross with Nd-0; Mark Daly for excellent assistance with the data analysis; Stanley Tamaki, Bonnie Pruitt, and Patty Pang for assistance with plant DNA preparations; Sherry Kempin for assistance with gel blots and phage DNA preparations; and members of the laboratory of E.M.M. for comments on the manuscript. This work was supported by a National Science Foundation grant (DCB-870349) to E.M.M. J.L.B. was supported, and C.C. was partially supported, by a National Institutes of Health training grant (5T32-GM07616). E.S.L. was partially supported by a National Science Foundation grant (DCB-8611317) and by a System Development Foundation grant (G612). The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact.
Subject Keywords:genetic map, Brassicaceae
Record Number:CaltechAUTHORS:CHApnas88
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:CHApnas88
Alternative URL:http://www.pnas.org/cgi/content/abstract/85/18/6856
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:657
Collection:CaltechAUTHORS
Deposited By: Archive Administrator
Deposited On:12 Sep 2005
Last Modified:14 Nov 2014 19:18

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