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Molecular Cloning and DNA Sequence of the Arabidopsis thaliana Alcohol Dehydrogenase Gene

Chang, Caren and Meyerowitz, Eliott M. (1986) Molecular Cloning and DNA Sequence of the Arabidopsis thaliana Alcohol Dehydrogenase Gene. Proceedings of the National Academy of Sciences of the United States of America, 83 (5). pp. 1408-1412. ISSN 0027-8424. http://resolver.caltech.edu/CaltechAUTHORS:CHApnas86

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Abstract

Arabidopsis thaliana provides an excellent experimental plant system for molecular genetics because of its remarkably small genome size, near absence of dispersed middle repetitive DNA, and short life cycle. We have cloned and determined the nucleotide sequence of a single-copy gene from A. thaliana likely to be the gene encoding alcohol dehydrogenase (ADH; alcohol:NAD+ oxidoreductase, EC 1.1.1.1). The gene was isolated from a random recombinant library by cross-hybridization with a maize Adh1 gene probe. The DNA sequence contains an open reading frame capable of encoding a polypeptide the same length as maize ADH1 and ADH2 (379 amino acids) and having approx 80% homology with both maize enzymes. This open reading frame is interrupted by six introns whose positions are conserved with six of the nine intron positions present in both maize genes. The 5' and 3' untranslated regions are, respectively, 58 and 204 base pairs long. Sequences important for eukaryotic gene expression such as the TATA box, polyadenylylation signal, and intron splicesite sequences are found in the expected locations. The gene hybridizes to a specific anaerobically induced RNA in Arabidopsis whose appearance correlates with the anaerobic induction of Arabidopsis ADH protein.


Item Type:Article
Additional Information:Copyright © 1986 by the National Academy of Sciences Communicated by Ray D. Owen, October 18, 1985. We are grateful to C. Rice for technical advice and to T. Hunkapiller and C. Martin forDNA sequence analysis programs. We thank M. Garfinkel, P. Mathers, K. Fryxell, and R. Pruitt for comments on the manuscript. This work was supported by Grant PCM-8408504 from the National Science Foundation (E.M.M.). C.C. was supported by National Research Service Award 5 T32GM07616 from the National Institutes of Health. The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact.
Subject Keywords:gene structure, enzyme Induction, intron, evolution
Record Number:CaltechAUTHORS:CHApnas86
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:CHApnas86
Alternative URL:http://www.pnas.org/cgi/content/abstract/83/5/1408
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:659
Collection:CaltechAUTHORS
Deposited By: Archive Administrator
Deposited On:12 Sep 2005
Last Modified:26 Dec 2012 08:40

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