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Distinct Single-Copy Sequence Sets in Sea Urchin Nuclear RNAs

Ernst, Susan G. and Britten, Roy J. and Davidson, Eric H. (1979) Distinct Single-Copy Sequence Sets in Sea Urchin Nuclear RNAs. Proceedings of the National Academy of Sciences of the United States of America, 76 (5). pp. 2209-2212. ISSN 0027-8424. http://resolver.caltech.edu/CaltechAUTHORS:ERNpnas79

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Abstract

The purpose of this study was to determine whether nuclear RNAs (nRNA) of sea urchin embryos and adult tissues contain identical or partially distinct sets of single-copy sequence transcripts. A DNA tracer was prepared consisting mainly of sequences absent from gastrula nRNA; 3.6% of this tracer reacted with adult intestine nRNA but not with gastrula nRNA. The existence of a differentially transcribed DNA fraction was verified by its partial purification and rehybridization to intestine and gastrula nRNAs. About one-third of the genomic single-copy sequence is represented in both nRNAs, or about 2 x 10^8 nucleotides. The differentially transcribed portion of the single-copy genome identified in this work includes about 3.5 x 10^7 nucleotides.


Item Type:Article
Additional Information:Copyright © 1979 by the National Academy of Sciences Contributed by Roy J. Britten, February 21, 1979 We thank Mr. Clifford Beall for technical assistance. This research was supported by grants from the National Institutes of Health, HD-05753 and RR-07003 (awarded by the Biomedical Research Support Grant Program). S.G.E. was supported by National Institutes of Health Fellowship GM-05273. The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact.
Subject Keywords:complexity; embryo nuclear RNA
Record Number:CaltechAUTHORS:ERNpnas79
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:ERNpnas79
Alternative URL:http://www.pnas.org/cgi/content/abstract/76/5/2209
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:6637
Collection:CaltechAUTHORS
Deposited By: Archive Administrator
Deposited On:16 Dec 2006
Last Modified:14 Nov 2014 19:19

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