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The effect of 3-indoleacetic acid on the response of Lactobacillus arabinosus 17-5 to nicotinamide

Galston, Arthur W. and Hand, Margery E. (1949) The effect of 3-indoleacetic acid on the response of Lactobacillus arabinosus 17-5 to nicotinamide. Journal of Biological Chemistry, 178 (2). pp. 967-970. ISSN 0021-9258. http://resolver.caltech.edu/CaltechAUTHORS:GALjbc49

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Abstract

Lactobacillus arabinosus 17-5 has been widely used as an assay organism for nicotinic acid (NA) since the development of the method by Snell and Wright (1). Although it has been realized that other substances present in tissue extracts may interfere with the bioassay, the nature of such substances has not been elucidated. During an investigation of tryptophan metabolism in the pea plant, we studied the possible conversion of this compound to nicotinic acid, since such a transformation has been demonstrated to occur in numerous organisms (2-4). The method involved infiltration of tissue with large quantities of tryptophan and subsequent bioassay for nicotinic acid with Lactobacillus arabinosus 17-5. Certain anomalous results led us to believe that other metabolites of tryptophan were interfering with the assay. Because 3-indoleacetic acid (IAA) is a known plant metabolite of tryptophan (5), we tested it for possible interference with the assay, and, as described below, found that such interference may occur under certain circumstances.


Item Type:Article
Additional Information:Copyright © 1949 by the American Society for Biochemistry and Molecular Biology. (Received for publication, November 15, 1948)
Record Number:CaltechAUTHORS:GALjbc49
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:GALjbc49
Alternative URL:http://www.jbc.org/content/vol178/issue2/index.shtml
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:6645
Collection:CaltechAUTHORS
Deposited By: Archive Administrator
Deposited On:16 Dec 2006
Last Modified:26 Dec 2012 09:23

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