Kanamori, Keiko and Weiss, Richard L. and Roberts, John D. (1987) Role of glutamate dehydrogenase in ammonia assimilation in nitrogen-fixing Bacillus macerans. Journal of Bacteriology, 169 (10). pp. 4692-4695. ISSN 0021-9193 http://resolver.caltech.edu/CaltechAUTHORS:KANjbact87
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Pathways of ammonia assimilation into glutamic acid in Bacillus macerans were investigated by measurements of the specific activities of glutamate dehydrogenase (GDH), glutamine synthetase, and glutamate synthase. In ammonia-rich medium, GDH was the predominant pathway of ammonia assimilation. In nitrogen-fixing cells in which the intracellular NH4+ concentration was 1.4 +/- 0.5 mM, the activity of GDH with a Km of 2.2 mM for NH4+ was found to be severalfold higher than that of glutamate synthase. The result suggests that GDH plays a significant role in the assimilation of NH4+ in N2-fixing B. macerans.
|Additional Information:||Copyright © 1987 by the American Society for Microbiology. Received 23 March 1987/Accepted 17 July 1987 This work was supported by National Science Foundation grant DMB85-01617. Contribution no. 7563 from the Gates and Crellin Laboratories of Chemistry, California Institute of Technology.|
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|Deposited On:||18 Dec 2006|
|Last Modified:||26 Dec 2012 09:24|
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