Kanamori, Keiko and Weiss, Richard L. and Roberts, John D. (1987) Role of glutamate dehydrogenase in ammonia assimilation in nitrogen-fixing Bacillus macerans. Journal of Bacteriology, 169 (10). pp. 4692-4695. ISSN 0021-9193 http://resolver.caltech.edu/CaltechAUTHORS:KANjbact87
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Abstract
Pathways of ammonia assimilation into glutamic acid in Bacillus macerans were investigated by measurements of the specific activities of glutamate dehydrogenase (GDH), glutamine synthetase, and glutamate synthase. In ammonia-rich medium, GDH was the predominant pathway of ammonia assimilation. In nitrogen-fixing cells in which the intracellular NH4+ concentration was 1.4 +/- 0.5 mM, the activity of GDH with a Km of 2.2 mM for NH4+ was found to be severalfold higher than that of glutamate synthase. The result suggests that GDH plays a significant role in the assimilation of NH4+ in N2-fixing B. macerans.
| Item Type: | Article |
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| Additional Information: | Copyright © 1987 by the American Society for Microbiology. Received 23 March 1987/Accepted 17 July 1987 This work was supported by National Science Foundation grant DMB85-01617. Contribution no. 7563 from the Gates and Crellin Laboratories of Chemistry, California Institute of Technology. |
| Record Number: | CaltechAUTHORS:KANjbact87 |
| Persistent URL: | http://resolver.caltech.edu/CaltechAUTHORS:KANjbact87 |
| Alternative URL: | http://jb.asm.org/cgi/content/abstract/169/10/4692 |
| Usage Policy: | No commercial reproduction, distribution, display or performance rights in this work are provided. |
| ID Code: | 6696 |
| Collection: | CaltechAUTHORS |
| Deposited By: | Archive Administrator |
| Deposited On: | 18 Dec 2006 |
| Last Modified: | 26 Dec 2012 09:24 |
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