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Role of glutamate dehydrogenase in ammonia assimilation in nitrogen-fixing Bacillus macerans

Kanamori, Keiko and Weiss, Richard L. and Roberts, John D. (1987) Role of glutamate dehydrogenase in ammonia assimilation in nitrogen-fixing Bacillus macerans. Journal of Bacteriology, 169 (10). pp. 4692-4695. ISSN 0021-9193. http://resolver.caltech.edu/CaltechAUTHORS:KANjbact87

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Abstract

Pathways of ammonia assimilation into glutamic acid in Bacillus macerans were investigated by measurements of the specific activities of glutamate dehydrogenase (GDH), glutamine synthetase, and glutamate synthase. In ammonia-rich medium, GDH was the predominant pathway of ammonia assimilation. In nitrogen-fixing cells in which the intracellular NH4+ concentration was 1.4 +/- 0.5 mM, the activity of GDH with a Km of 2.2 mM for NH4+ was found to be severalfold higher than that of glutamate synthase. The result suggests that GDH plays a significant role in the assimilation of NH4+ in N2-fixing B. macerans.


Item Type:Article
Additional Information:Copyright © 1987 by the American Society for Microbiology. Received 23 March 1987/Accepted 17 July 1987 This work was supported by National Science Foundation grant DMB85-01617. Contribution no. 7563 from the Gates and Crellin Laboratories of Chemistry, California Institute of Technology.
Record Number:CaltechAUTHORS:KANjbact87
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:KANjbact87
Alternative URL:http://jb.asm.org/cgi/content/abstract/169/10/4692
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:6696
Collection:CaltechAUTHORS
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Deposited On:18 Dec 2006
Last Modified:26 Dec 2012 09:24

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