Published November 8, 1989
| public
Journal Article
Nonenzymatic ligation of oligodeoxyribonucleotides on a duplex DNA template by triple-helix formation
- Creators
- Luebke, Kevin J.
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Dervan, Peter B.
Chicago
An error occurred while generating the citation.
Abstract
We report that a double-stranded DNA template can direct the sequence-specific formation of a phosphodiester linkage between oligodeoxyribonucleotides in aqueous solution by juxtaposing the termini head-to-tail in a triple-helical complex (Figure 1). A triple-stranded complex can be formed when a pyrimidine oligodeoxynucleotide binds in the major groove of duplex DNA parallel to the purine Watson-Crick strand. In the complex, sequence specificity for the condensation of oligodeoxynucleotides is directed by Hoogsteen base pairing.
Additional Information
© 1989 American Chemical Society. Received August 2, 1989. This work was supported in part by the National Institutes of Health (GM-35724) and the Caltech Consortium in Chemistry and Chemical Engineering (founding members: E. I. du Pont de Nemours and Company, Inc., Eastman Kodak Company, Minnesota Mining and Manufacturing Company, and Shell Oil Company Foundation). We are grateful for a National Research Service Award to K.J.L. from the National Institute of General Medical Science.Additional details
- Eprint ID
- 66961
- DOI
- 10.1021/ja00205a033
- Resolver ID
- CaltechAUTHORS:20160510-152740060
- NIH
- GM-35724
- Caltech Consortium in Chemistry and Chemical Engineering
- NIH Predoctoral Fellowship
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- Updated
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