Kanamori, Keiko and Legerton, Timothy L. and Weiss, Richard L. and Roberts, John D. (1982) Effect of the nitrogen source on glutamine and alanine biosynthesis in Neurospora crassa. An in vivo 15N nuclear magnetic resonance study. Journal of Biological Chemistry, 257 (23). pp. 14168-14172. ISSN 0021-9258. http://resolver.caltech.edu/CaltechAUTHORS:KANjbc82
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The influences of different nitrogen sources on the relative rates of biosynthesis of glutamine and alanine have been studied by 15N nuclear magnetic resonance spectroscopy of intact Neurospora crassa mycelia suspensions. The rate of glutamine synthesis was fastest after growth in media deficient in free ammonium ion, whereas it was slowest following growth in media containing both glutamic acid and glutamine. The reverse trend was observed for the biosynthesis of alanine. A competition between the two biosynthetic pathways for the same substrate, glutamic acid, was found to limit the rate of alanine synthesis when glutamine synthesis was rapid. The observed in vivo rates of these reactions are compared to the reported specific activities of the enzymes catalyzing the reactions, and implications of these results for nitrogen regulation of these pathways under various physiological conditions are discussed.
|Additional Information:||Copyright © 1982 by the American Society for Biochemistry and Molecular Biology. (Received for publication, February 26, 1982) [J.D.R. was] [s]upported by the National Science Foundation and by United States Public Health Service Research Grant GM-11072 from the Division of General Medical Sciences. Contribution 6610 from the Gates and Crellin Laboratories of Chemistry, California Institute of Technology. [T.L.L. was] [s]upported by United States Public Health Service National Research Award 05104 [and ...] by National Science Foundation Grant PCM 78-19030. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked “advertisement” in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.|
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|Deposited On:||19 Dec 2006|
|Last Modified:||26 Dec 2012 09:24|
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