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Identification of Yeast Mutants with Altered Telomere Structure

Lustig, Arthur J. and Petes, Thomas D. (1986) Identification of Yeast Mutants with Altered Telomere Structure. Proceedings of the National Academy of Sciences of the United States of America, 83 (5). pp. 1398-1402. ISSN 0027-8424. http://resolver.caltech.edu/CaltechAUTHORS:LUSpnas86

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Abstract

The chromosomes of the yeast Saccharomyces cerevisiae terminate in a tract of simple-sequence DNA [poly(C1-3A)] that is several hundred base pairs long. We describe the identification of mutant yeast strains that have telomeric tracts that are shorter than normal. A genetic analysis of these strains indicates that these short telomeres are the result of single nuclear recessive mutations and that these mutations can be classified into two different complementation groups. The full expression of the mutant phenotype shows a very long lag (approx 150 cell divisions). From our analysis of these mutants as well as other data, we suggest that the duplication of the telomeric poly(C1-3A) tract involves two processes, semiconservative replication and untemplated terminal addition of nucleotides.


Item Type:Article
Additional Information:Copyright © 1986 by the National Academy of Sciences Communicated by John Abelson, October 9, 1985 We thank Drs. Bik Tye, Mark Krassner, Mark Goebl, Richard Walmsley, Mike Carson, and Lee Hartwell for discussions concerning telomere replication and Drs. Sternglanz, McLaughlin, Carson, and Hartwell for providing several of the strains used in this study. We thank Michael Wing and Dorothy Stamenkovich for technical assistance and Drs. Tye, Jinks-Robertson, and Symington for their comments on this manuscript. The research was supported by grants from the National Institutes of Health (GM24110 and K-04-AG-007) and the National Cancer Institute (CA19265). The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact.
Subject Keywords:DNA replication; phenotypic lag; simple-sequence DNA
Record Number:CaltechAUTHORS:LUSpnas86
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:LUSpnas86
Alternative URL:http://www.pnas.org/cgi/content/abstract/83/5/1398
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:6748
Collection:CaltechAUTHORS
Deposited By: Archive Administrator
Deposited On:20 Dec 2006
Last Modified:14 Nov 2014 19:19

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