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Two Regulatory Elements for Immunoglobulin kappa Light Chain Gene Expression

Bergman, Yehudit and Rice, Douglas and Grosschedl, Rudolf and Baltimore, David (1984) Two Regulatory Elements for Immunoglobulin kappa Light Chain Gene Expression. Proceedings of the National Academy of Sciences of the United States of America, 81 (22). pp. 7041-7045. ISSN 0027-8424. http://resolver.caltech.edu/CaltechAUTHORS:BERpnas84

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Abstract

By using internal deletions within a rearranged immunoglobulin kappa light chain gene, the presence of an intron regulatory sequence (enhancer) has been confirmed. Its presence is required for high-level transcription from a plasmid after transfection into myeloma cells. Transfection efficiency was monitored by the activity of a deleted H4 histone gene included in the plasmid. The intron element could be moved upstream of the gene in both orientations, fulfilling the definition of an enhancer. By using 5' deletions, a second regulatory element was located upstream of the "TATA" box, between positions -69 and -104. These two elements both are required for efficient kappa chain gene expression.


Item Type:Article
Additional Information:Copyright © 1984 by the National Academy of Sciences Contributed by David Baltimore, July 30, 1984 This work was supported by American Cancer Society Grant IM-355P. Y.B. was supported by a Bantrell Postdoctoral Fellowship. D.R. was supported by a Helen Hay Whitney Postdoctoral Fellowship. R.G. was supported by an European Molecular Biology Organization Fellowship. The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact.
Subject Keywords:transfections; enhancers; promoters
Record Number:CaltechAUTHORS:BERpnas84
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:BERpnas84
Alternative URL:http://www.pnas.org/cgi/content/abstract/81/22/7041
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:6911
Collection:CaltechAUTHORS
Deposited By: Archive Administrator
Deposited On:02 Jan 2007
Last Modified:26 Dec 2012 09:26

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