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Molecular basis of heavy-chain class switching and switch region deletion in an Abelson virus-transformed cell line

DePinho, Ronald and Kruger, Kelly and Andrews, Nancy and Lutzker, Stuart and Baltimore, David and Alt, Frederick W. (1984) Molecular basis of heavy-chain class switching and switch region deletion in an Abelson virus-transformed cell line. Molecular and Cellular Biology, 4 (12). pp. 2905-2910. ISSN 0270-7306.

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We demonstrated that a subclone of an Abelson murine leukemia virus-transformed B-lymphoid cell line switched from mu to gamma 2b expression in vitro, by the classical recombination-deletion mechanism. In this line, the expressed VHDJH region and the C gamma 2b constant region gene were juxtaposed by a recombination event which linked the highly repetitive portions of the S mu and S gama 2b regions and resulted in the loss of the C mu gene from the intervening region. An additional recombination event in this subclone involved an internal deletion in the S mu region of the expressed (switched) allele. One end of this deletion occurred very close to the switch recombination point. Despite the recombination-deletion mechanism of switching, the gamma 2b-producing line retained two copies of the C mu gene and two copies of the sequence just 5' to the S gamma 2b recombination point. The possible significance of the retention of these sequences to the mechanism of class switching is discussed.

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Additional Information:Copyright © 1984 by the American Society for Microbiology. Received 13 June 1984/Accepted 25 September 1984 We thank K. Marcu for providing the SL1 clone from which the 5'- and 3'-Sγ2b probes were derived and K. Blackwell for critically reading this manuscript. This work was supported by American Cancer Society grants NP-393 (F.W.A.) and MV-34M (D.B.). F.W.A. is a recipient of an Irma T. Hirschl Career Scientist Award.
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Deposited On:02 Jan 2007
Last Modified:26 Dec 2012 09:26

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