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The site of synthesis of the iron-sulfur subunits of the flavoprotein and iron-protein fractions of human NADH dehydrogenase

Chomyn, Anne and Patel, Salil D. and Cleeter, Michael W. J. and Ragan, C. Ian and Attardi, Giuseppe (1988) The site of synthesis of the iron-sulfur subunits of the flavoprotein and iron-protein fractions of human NADH dehydrogenase. Journal of Biological Chemistry, 263 (31). pp. 16395-16400. ISSN 0021-9258. http://resolver.caltech.edu/CaltechAUTHORS:CHOjbc88

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Abstract

The site of synthesis of the iron-sulfur subunits of the flavoprotein and iron-protein fractions of the human respiratory chain NADH dehydrogenase has been investigated to test the possibility that any of them is synthesized in mitochondria. For this purpose, antibodies specific for individual subunits of the bovine enzyme, which cross- reacted with the homologous human subunits in immunoblot assays, were tested against HeLa cell mitochondrial proteins labeled in vivo with [35S]methionine in the absence or presence of inhibitors of mitochondrial or cytoplasmic protein synthesis. The results clearly indicated that all the iron-sulfur subunits of the flavoprotein and iron-protein fractions of human complex I are synthesized in the cytosol and are, therefore, encoded in nuclear genes.


Item Type:Article
Additional Information:Copyright © 1988 by the American Society for Biochemistry and Molecular Biology. (Received for publication, April 6, 1988) The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked “advertisement” in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
Record Number:CaltechAUTHORS:CHOjbc88
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:CHOjbc88
Alternative URL:http://www.jbc.org/cgi/content/abstract/263/31/16395
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:6916
Collection:CaltechAUTHORS
Deposited By: Archive Administrator
Deposited On:02 Jan 2007
Last Modified:26 Dec 2012 09:26

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