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A Poliovirus Temperature-Sensitive RNA Synthesis Mutant Located in a Noncoding Region of the Genome

Sarnow, Peter and Bernstein, Harris D. and Baltimore, David (1986) A Poliovirus Temperature-Sensitive RNA Synthesis Mutant Located in a Noncoding Region of the Genome. Proceedings of the National Academy of Sciences of the United States of America, 83 (3). pp. 571-575. ISSN 0027-8424. http://resolver.caltech.edu/CaltechAUTHORS:SARpnas86

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Abstract

We have constructed an 8-base-pair insertion mutation in the 3' noncoding region of an infectious poliovirus cDNA clone that gives rise to a temperature-sensitive RNA synthesis mutant upon transfection into mammalian cells. The mutated cDNA was used to establish a cell line that releases the mutant poliovirus in a temperature-dependent fashion, representing a unique persistent viral infection. A poliovirus mutant mapping in the noncapsid region of the viral genome can be complemented in this cell line, implying that the cell line expresses viral proteins at the nonpermissive temperature.


Item Type:Article
Additional Information:Copyright © 1986 by the National Academy of Sciences Contributed by David Baltimore, September 9, 1985 We thank Karla Kirkegaard for many valuable suggestions during the course of this work and for helpful comments on the manuscript. We also thank Mike Gilman for critical reading of this manuscript. This work was supported by grants from the National Institute for Allergy and Infectious Diseases. P.S. was supported by a postdoctoral fellowship from the Deutsche Forschungsgemeinschaft. The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact.
Subject Keywords:poliovirus cDNA; site-directed mutagenesis; persistent viral infection; complementation
Record Number:CaltechAUTHORS:SARpnas86
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:SARpnas86
Alternative URL:http://www.pnas.org/cgi/content/abstract/83/3/571
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:6944
Collection:CaltechAUTHORS
Deposited By: Archive Administrator
Deposited On:03 Jan 2007
Last Modified:14 Nov 2014 19:19

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