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Members of the methanotrophic genus Methylomarinum inhabit inland mud pots

Fradet, Danielle T. and Tavormina, Patricia L. and Orphan, Victoria J. (2016) Members of the methanotrophic genus Methylomarinum inhabit inland mud pots. PeerJ, 4 . Art. No. e2116. ISSN 2167-8359. PMCID PMC4950536. http://resolver.caltech.edu/CaltechAUTHORS:20160811-151334696

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[img] Image (JPEG) (Figure S1 - Sites sampled in the Davis-Schrimpf seep field in 2012 and 2016) - Supplemental Material
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[img] Image (JPEG) (Figure S2 - Appearance of Methylococcaceae strain SSMP-1 when grown on solid medium) - Supplemental Material
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[img] Image (JPEG) (Figure S3 - Water levels at Site 2 in the Davis Schrimpf seep field in 2012 and 2016.) - Supplemental Material
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[img] Video (QuickTime) (Movie S1 - Motility in Methylococcaceae strain SSMP-1, video loop) - Supplemental Material
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Abstract

Proteobacteria capable of converting the greenhouse gas methane to biomass, energy, and carbon dioxide represent a small but important sink in global methane inventories. Currently, 23 genera of methane oxidizing (methanotrophic) proteobacteria have been described, although many are represented by only a single validly described species. Here we describe a new methanotrophic isolate that shares phenotypic characteristics and phylogenetic relatedness with the marine methanotroph Methylomarinum vadi. However, the new isolate derives from a terrestrial saline mud pot at the northern terminus of the Eastern Pacific Rise (EPR). This new cultivar expands our knowledge of the ecology of Methylomarinum, ultimately towards a fuller understanding of the role of this genus in global methane cycling.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.7717/peerj.2116DOIArticle
https://peerj.com/articles/2116/PublisherArticle
http://dx.doi.org/10.7717/peerj.2116/supp-1 DOIFigure S1
http://dx.doi.org/10.7717/peerj.2116/supp-2 DOIFigure S2
http://dx.doi.org/10.7717/peerj.2116/supp-3 DOIFigure S3
http://dx.doi.org/10.7717/peerj.2116/supp-4 DOIFigure S4
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4950536/PubMed CentralArticle
ORCID:
AuthorORCID
Orphan, Victoria J.0000-0002-5374-6178
Additional Information:© 2016 Fradet et al. Distributed under Creative Commons CC-BY 4.0. Submitted 25 February 2016; Accepted 18 May 2016; Published 12 July 2016. We thank Roland Hatzenpichler and Derek Smith for sampling the Salton Sea mudpots in 2012 and providing critical methodological, graphic, and scientific input.We thank Alyssa Boedigheimer for assistance in sample collection in January 2016. We thank Stephanie Connon for assistance with the 16S rRNA gene phylogeny. Funding for this work was provided by the Gordon and Betty Moore Foundation, in a grant to Victoria J. Orphan (grant no. GBMF3780). This research was additionally supported by a grant from the NASA Astrobiology Institute (Award # NNA13AA92A). This is NAI-Life Underground Publication Number 083. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Grant Disclosures: The following grant information was disclosed by the authors: Gordon and Betty Moore Foundation, in a grant to Victoria J. Orphan: GBMF3780. NASA Astrobiology Institute, in a grant to Victoria J. Orphan: NNA13AA92A. The authors declare that they have no competing interests. Author Contributions: Danielle T. Fradet conceived and designed the experiments, performed the experiments, analyzed the data, wrote the paper, reviewed drafts of the paper. Patricia L. Tavormina conceived and designed the experiments, analyzed the data, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper. Victoria J. Orphan conceived and designed the experiments, wrote the paper, reviewed drafts of the paper, intellectual Support, encouragement, and funding.
Funders:
Funding AgencyGrant Number
Gordon and Betty Moore FoundationGBMF3780
NASANNA13AA92A
Subject Keywords:Methanotroph, Microbial ecology, Eastern Pacific Rise, Davis-Schrimpf seep field, pmoA, Bacterial methane oxidizers
PubMed Central ID:PMC4950536
Record Number:CaltechAUTHORS:20160811-151334696
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20160811-151334696
Official Citation:Fradet DT, Tavormina PL, Orphan VJ. (2016) Members of the methanotrophic genus Methylomarinum inhabit inland mud pots. PeerJ 4:e2116 https://doi.org/10.7717/peerj.2116
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:69572
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:11 Aug 2016 23:23
Last Modified:11 Aug 2016 23:23

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