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Molecular Cloning of cDNA for the Nuclear Ribonucleoprotein Particle C Proteins: A Conserved Gene Family

Nakagawa, Terry Y. and Swanson, Maurice S. and Wold, Barbara J. and Dreyfuss, Gideon (1986) Molecular Cloning of cDNA for the Nuclear Ribonucleoprotein Particle C Proteins: A Conserved Gene Family. Proceedings of the National Academy of Sciences of the United States of America, 83 (7). pp. 2007-2011. ISSN 0027-8424. http://resolver.caltech.edu/CaltechAUTHORS:NAKpnas86

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Abstract

The C proteins, C1 and C2, are major constituents of the heterogeneous nuclear RNA (hnRNA) ribonucleoprotein (hnRNP) complex in vertebrates. C1 and C2 are antigenically related phosphoproteins that are in contact with hnRNA in intact cells and bind to RNA tightly in vitro. A cDNA clone for the C proteins was isolated by immunological screening of a human lambda gt11 expression vector cDNA library with monoclonal antibodies. The lacZ-cDNA fusion protein is recognized by two different anti-C protein monoclonal antibodies. HeLa cell mRNA that was hybrid-selected with the cDNA clone (1.1 kilobases long) was translated in vitro and yielded both the C1 and C2 proteins (41 and 43 kDa, respectively). RNA blot analysis showed strong hybridization to two polyadenylylated transcripts, of about 1.4 kb and 1.9 kb, in human cells. Genomic DNA blot analysis showed multiple hybridizing restriction fragments in human and mouse, and homologous DNA sequences are found across eukaryotes from human to yeast. These findings suggest that the sequences encoding the hnRNP C proteins are members of a conserved gene family and they open the way for detailed molecular and genetic studies of these proteins.


Item Type:Article
Additional Information:Copyright © 1986 by the National Academy of Sciences Communicated by E. Margoliash, November 15, 1985. We thank Masayuki Yamamoto for helpful advice on the lysogen preparation. This work was supported by grants from the Rita Allen Foundation and the Searle Scholars Program to B.J.W. and from the National Institutes of Health (GM31888) and the Searle Leadership Fund to G.D. The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact.
Subject Keywords:heterogeneous nuclear RNA packaging, RNA splicing, monoclonal antibodies, [lambda]gt11 expression vector
Record Number:CaltechAUTHORS:NAKpnas86
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:NAKpnas86
Alternative URL:http://www.pnas.org/cgi/content/abstract/83/7/2007
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:703
Collection:CaltechAUTHORS
Deposited By: Archive Administrator
Deposited On:15 Sep 2005
Last Modified:26 Dec 2012 08:41

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