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Introduction and Expression of a Rabbit ß-globin Gene in Mouse Fibroblasts

Wold, B. and Wigler, M. and Lacy, E. and Maniatis, T. and Silverstein, S. and Axel, R. (1979) Introduction and Expression of a Rabbit ß-globin Gene in Mouse Fibroblasts. Proceedings of the National Academy of Sciences of the United States of America, 76 (11). pp. 5684-5688. ISSN 0027-8424. http://resolver.caltech.edu/CaltechAUTHORS:WOLpnas79

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Abstract

The cloned chromosomal rabbit ß-globin gene has been introduced into mouse fibroblasts by DNA-mediated gene transfer (transformation). In this report, we examine the expression of the rabbit gene in six independent transformants that contain from 1 to 20 copies of the cloned globin gene. Rabbit globin transcripts were detected in two of these transformants at steady-state concentrations of 5 and 2 copies per cell. The globin transcripts from one cell line are polyadenylylated and migrate as 9S RNA on methylmercury gels. These transcripts reflect correct processing of the two intervening sequences but lack 48 ± 5 nucleotides present at the 5' terminus of rabbit erythrocyte globin mRNA.


Item Type:Article
Additional Information:Copyright © 1979 by the National Academy of Sciences Communicated by Sol Spiegelman, July 16, 1979. The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U. S. C. §1734 solely to indicate this fact.
Subject Keywords:DNA-mediated gene transfer, cotransformation, intervening sequences, gene regulation
Record Number:CaltechAUTHORS:WOLpnas79
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:WOLpnas79
Alternative URL:http://www.pnas.org/cgi/content/abstract/76/11/5684
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:704
Collection:CaltechAUTHORS
Deposited By: Archive Administrator
Deposited On:15 Sep 2005
Last Modified:26 Dec 2012 08:41

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