Wieland, Thomas and Bahitjari, Nehat and Zhou, Xiao-Bo and Kleuss, Christiane and Simon, Melvin I. (2000) Polarity Exchange at the Interface of Regulators of G Protein Signaling with G Protein α-Subunits. Journal of Biological Chemistry, 275 (37). pp. 28500-28506. ISSN 0021-9258. http://resolver.caltech.edu/CaltechAUTHORS:WIEjbc00
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RGS proteins are GTPase-activating proteins (GAPs) for G protein alpha -subunits. This GAP activity is mediated by the interaction of conserved residues on regulator of G protein signaling (RGS) proteins and Galpha -subunits. We mutated the important contact sites Glu-89, Asn-90, and Asn-130 in RGS16 to lysine, aspartate, and alanine, respectively. The interaction of RGS16 and its mutants with Galpha t and Galpha i1 was studied. The GAP activities of RGS16N90D and RGS16N130A were strongly attenuated. RGS16E89K increased GTP hydrolysis of Galpha i1 by a similar extent, but with an about 100-fold reduced affinity compared with non-mutated RGS16. As Glu-89 in RGS16 is interacting with Lys-210 in Galpha i1, this lysine was changed to glutamate for compensation. Galpha i1K210E was insensitive to RGS16 but interacted with RGS16E89K. In rat uterine smooth muscle cells, wild type RGS16 abolished Gi-mediated alpha 2-adrenoreceptor signaling, whereas RGS16E89K was without effect. Both Galpha i1 and Galpha i1K210E mimicked the effect of alpha 2-adrenoreceptor stimulation. Galpha i1K210E was sensitive to RGS16E89K and 10-fold more potent than Galpha i1. Analogous mutants of Galpha q (Galpha qK215E) and RGS4 (RGS4E87K) were created and studied in COS-7 cells. The activity of wild type Galpha q was counteracted by wild type RGS4 but not by RGS4E87K. The activity of Galpha qK215E was inhibited by RGS4E87K, whereas non-mutated RGS4 was ineffective. We conclude that mutation of a conserved lysine residue to glutamate in Galpha i and Galpha q family members renders these proteins insensitive to wild type RGS proteins. Nevertheless, they are sensitive to glutamate to lysine mutants of RGS proteins. Such mutant pairs will be helpful tools in analyzing Galpha -RGS specificities in living cells.
|Additional Information:||© 2000 The American Society for Biochemistry and Molecular Biology, Inc. Received for publication, May 16, 2000, and in revised form, June 20, 2000. Originally published In Press as doi:10.1074/jbc.M004187200 on June 30, 2000. We thank Dr. J. H. Kehrl (National Institutes of Health, Bethesda) for providing the RGS4 expression vector pCR3-RGS4. The expert technical assistance of Grit Höppner is greatly appreciated. This work was supported by the Deutsche Forschungsgemeinschaft.The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.|
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|Deposited On:||05 Jan 2007|
|Last Modified:||31 Mar 2017 18:49|
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