Peck, Lawrence J. and Millstein, Larry and Eversole-Cire, Pamela and Gottesfeld, Joel M. and Varshavsky, Alexander (1987) Transcriptionally inactive oocyte-type 5S RNA genes of Xenopus laevis are complexed with TFIIIA in vitro. Molecular and Cellular Biology, 7 (10). pp. 3503-3510. ISSN 0270-7306. http://resolver.caltech.edu/CaltechAUTHORS:PECmcb87
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An extract from whole oocytes of Xenopus laevis was shown to transcribe somatic-type 5S RNA genes approximately 100-fold more efficiently than oocyte-type 5S RNA genes. This preference was at least 10-fold greater than the preference seen upon microinjection of 5S RNA genes into oocyte nuclei or upon in vitro transcription in an oocyte nuclear extract. The approximately 100-fold transcriptional bias in favor of the somatic-type 5S RNA genes observed in vitro in the whole oocyte extract was similar to the transcriptional bias observed in developing Xenopus embryos. We also showed that in the whole oocyte extract, a promoter-binding protein required for 5S RNA gene transcription, TFIIIA, was bound both to the actively transcribed somatic-type 5S RNA gene and to the largely inactive oocyte-type 5S RNA genes. These findings suggest that the mechanism for the differential expression of 5S RNA genes during Xenopus development does not involve differential binding of TFIIIA to 5S RNA genes.
|Additional Information:||Copyright © 1987 by the American Society for Microbiology. Received 27 March 1987/Accepted 3 July 1987 We thank D. Bogenhagen, D. Brown, D. Setzer, and J. Wang for recombinant DNA plasmids, J. Blanco for purified TFIIIA, and J.P. McGrath for comments on the manuscript. We also thank J. Schwager for his advice and gifts of numerous X. laevis. This work was supported by Public Health Service grants GM33401 and CA43309 from the National Institutes of Health to A.V. and grant GM26453 from the National Institute of General Medical Sciences and grant FRA292 from the American Cancer Society to J.G. L.P. was supported by a fellowship from the Damon Runyon-Walter Winchel Cancer Fund.|
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