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Persistence of Thymidine Kinase Activity in Mitochondria of a Thymidine Kinase-Deficient Derivative of Mouse L Cells

Attardi, Barbara and Attardi, Giuseppe (1972) Persistence of Thymidine Kinase Activity in Mitochondria of a Thymidine Kinase-Deficient Derivative of Mouse L Cells. Proceedings of the National Academy of Sciences of the United States of America, 69 (10). pp. 2874-2878. ISSN 0027-8424. http://resolver.caltech.edu/CaltechAUTHORS:ATTpnas72b

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Abstract

The cell line LM(TK-) Cl 1D, a derivative of mouse L fibroblasts deficient in thymidine kinase (EC 2.7.1.21) that shows very little thymidine kinase activity in extracts of whole cells as compared to the parental line, and that does not incorporate thymidine or 5-bromodeoxyuridine into nuclear DNA, has maintained the capacity to incorporate these precursors into mitochondrial DNA at a substantial rate. The amount of [methyl-3H]thymidine incorporated into mitochondrial DNA of LM(TK-) Cl 1D cells after long-term labeling has been conservatively estimated in different experiments to be between 30 and 60% of that incorporated into mitochondrial DNA or nuclear DNA of strain A9, an L-cell derivative without any thymidine-kinase deficiency; by contrast, the incorporatin of thymidine into nuclear DNA of Cl 1D cells is less than 1% of that in A9 cells. These results strongly suggest that the loss of thymidine kinase activity in the extramitochondrial compartment of LM(TK-) Cl 1D cells has not been accompanied by the loss of the mitochondria-associated enzyme activity, pointing to a different genetic or epigenetic control of the extramitochondrial and mitochondrial enzymes.


Item Type:Article
Additional Information:Copyright © 1972 by the National Academy of Sciences Communicated by Jerome Vinograd, July 31, 1972 After this manuscript was submitted for publication, a recent paper (23) by Clayton and Teplitz came to our attention in which they arrive at conclusions qualitatively, but not quantitatively, in agreement with ours. These investigations were supported by a research grant from the U.S. Public Health Service (GM-11726).
Subject Keywords:5-bromodeoxyuridine, ethidium bromide, mitochondrial DNA, density gradient centrifugation
Record Number:CaltechAUTHORS:ATTpnas72b
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:ATTpnas72b
Alternative URL:http://www.pnas.org/cgi/content/abstract/69/10/2874
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:719
Collection:CaltechAUTHORS
Deposited By: Archive Administrator
Deposited On:20 Sep 2005
Last Modified:14 Nov 2014 19:18

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