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RecA·Oligonucleotide Filaments Bind in the Minor Groove of Double-Stranded DNA

Baliga, Ramesh and Singleton, Jennifer W. and Dervan, Peter B. (1995) RecA·Oligonucleotide Filaments Bind in the Minor Groove of Double-Stranded DNA. Proceedings of the National Academy of Sciences of the United States of America, 92 (22). pp. 10393-10397. ISSN 0027-8424. http://resolver.caltech.edu/CaltechAUTHORS:BALpnas95

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Abstract

Escherichia coli RecA protein, in the presence of ATP or its analog adenosine 5'-[{gamma}-thio]triphosphate, polymerizes on single-stranded DNA to form nucleoprotein filaments that can then bind to homologous sequences on duplex DNA. The three-stranded joint molecule formed as a result of this binding event is a key intermediate in general recombination. We have used affinity cleavage to examine this three-stranded joint by incorporating a single thymidine-EDTA·Fe (T*) into the oligonucleotide part of the filament. Our analysis of the cleavage patterns from the joint molecule reveals that the nucleoprotein filament binds in the minor groove of an extended Watson-Crick duplex.


Item Type:Article
Additional Information:Copyright © 1995 by National Academy of Sciences Contributed by Peter B. Dervan, July 21, 1995 We thank Prof. S. C. Kowalczykowski for providing us with the RecA overexpressing strain JC12772 and a detailed protocol for isolation and purification, Amherst College for a Forris Jewett Moore Fellowship (J.W.S.), and the National Institutes of Health for grant support (GM-51747). The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact.
Record Number:CaltechAUTHORS:BALpnas95
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:BALpnas95
Alternative URL:http://www.pnas.org/cgi/content/abstract/92/22/10393
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:725
Collection:CaltechAUTHORS
Deposited By: Archive Administrator
Deposited On:21 Sep 2005
Last Modified:26 Dec 2012 08:41

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