Dirks, Robert M. and Pierce, Niles A. (2004) Triggered amplification by hybridization chain reaction. Proceedings of the National Academy of Sciences of the United States of America, 101 (43). pp. 15275-15278. ISSN 0027-8424 http://resolver.caltech.edu/CaltechAUTHORS:DIRpnas04
See Usage Policy.
Use this Persistent URL to link to this item: http://resolver.caltech.edu/CaltechAUTHORS:DIRpnas04
We introduce the concept of hybridization chain reaction (HCR), in which stable DNA monomers assemble only upon exposure to a target DNA fragment. In the simplest version of this process, two stable species of DNA hairpins coexist in solution until the introduction of initiator strands triggers a cascade of hybridization events that yields nicked double helices analogous to alternating copolymers. The average molecular weight of the HCR products varies inversely with initiator concentration. Amplification of more diverse recognition events can be achieved by coupling HCR to aptamer triggers. This functionality allows DNA to act as an amplifying transducer for biosensing applications.
|Additional Information:||© 2004 by The National Academy of Sciences of the USA From the cover. Communicated by Stephen L. Mayo, California Institute of Technology, Pasadena, CA, September 24, 2004 (received for review July 2, 2004). Published online before print October 18, 2004, 10.1073/pnas.0407024101 This work was supported by the Ralph M. Parsons Foundation and the Charles Lee Powell Foundation.|
|Usage Policy:||No commercial reproduction, distribution, display or performance rights in this work are provided.|
|Deposited By:||Archive Administrator|
|Deposited On:||21 Sep 2005|
|Last Modified:||26 Dec 2012 08:41|
Repository Staff Only: item control page