Hopfield, J. J. (1980) The energy relay: A proofreading scheme based on dynamic cooperativity and lacking all characteristic symptoms of kinetic proofreading in DNA replication and protein synthesis. Proceedings of the National Academy of Sciences of the United States of America, 77 (9). pp. 5248-5252. ISSN 0027-8424 http://resolver.caltech.edu/CaltechAUTHORS:HOPpnas80
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A mechanism for proofreading biosynthetic processes requiring high accuracy is described. The previously understood "kinetic proofreading" mechanism of enhancing accuracy has distinguishing characteristics such as the nonstoichiometric use of substrate or cosubstrate that have allowed its identification in aspects of DNA and protein synthesis. The proofreading scheme developed here, though generically related, lacks all the previous identifying features. A DNA polymerase proofreading in this manner need neither generate dNMP nor have a 3'→5' exonuclease activity. Protein synthesis could be proofread even with stoichiometric GTP consumption or without elongation factor Tu· GTP. The kinetic scheme that generates this proofreading makes use of an "energy relay" from previous substrate molecules and is a representative of a class of nonequilibrium processes displaying dynamic cooperativity. This proofreading mechanism has its own identifying characteristics, which are sufficiently subtle that they would have generally escaped notice or defied interpretation.
|Additional Information:||© 1980 by the National Academy of Sciences. Contributed by John J. Hopfield, June 16, 1980. I thank T. Yamane for interactions most helpful to this study and L.A. Loeb, M. Savageau, and M. Goodman for very helpful comments on the manuscript. The work at California Institute of Technology was supported in part by National Science Foundation Grant DMR8015678. This is contribution no. 6246 from the Division of Chemistry and Chemical Engineering. The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact.|
|Subject Keywords:||ribosomes; tRNA; accuracy; mutagenesis|
|Usage Policy:||No commercial reproduction, distribution, display or performance rights in this work are provided.|
|Deposited By:||Tony Diaz|
|Deposited On:||13 Feb 2007|
|Last Modified:||26 Dec 2012 09:31|
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