Emr, Scott D. and Silhavy, Thomas J. (1983) Importance of secondary structure in the signal sequence for protein secretion. Proceedings of the National Academy of Sciences of the United States of America, 80 (15). pp. 4599-4603. ISSN 0027-8424. http://resolver.caltech.edu/CaltechAUTHORS:EMRpnas83a
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Mutant Escherichia coli strains in which export of the LamB protein (coded for by the lamB gene) to the outer membrane of the cell is prevented have been described previously. One of these mutant strains contains a small (12-base pair) deletion mutation within the region of the lamB gene that codes for the NH2-terminal signal sequence. In this mutant strain, export but not synthesis of the LamB protein is blocked. We have isolated pseudorevertants that restore export of functional LamB protein to the outer membrane. DNA sequence analysis showed that two of the revertants contain a point mutation in addition to the original deletion. These point mutations lead to amino acid substitutions within the signal sequence. Our results indicate that these secondary mutations efficiently suppress the export defect caused by the deletion mutation. Analysis of the secondary structure of the wild-type, mutant, and pseudorevertant LamB signal sequences suggests that the secondary mutations restore export by allowing the formation of a stable α-helical conformation in the central, hydrophobic region of the signal sequence.
|Additional Information:||© 1983 by the National Academy of Sciences. Communicated by David Botstein, December 20, 1982. This work was sponsored by the National Cancer Institute, under Contract N01-CO-75380 with Litton Bionetics, Inc. The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact.|
|Subject Keywords:||LamB protein; export-defective mutants; pseudorevertants; DNA sequence analysis|
|Usage Policy:||No commercial reproduction, distribution, display or performance rights in this work are provided.|
|Deposited By:||Tony Diaz|
|Deposited On:||14 Feb 2007|
|Last Modified:||26 Dec 2012 09:31|
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