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Actin antibody: The specific visualization of actin filaments in non-muscle cells

Lazarides, Elias and Weber, Klaus (1974) Actin antibody: The specific visualization of actin filaments in non-muscle cells. Proceedings of the National Academy of Sciences of the United States of America, 71 (6). pp. 2268-2272. ISSN 0027-8424. http://resolver.caltech.edu/CaltechAUTHORS:LAZpnas74a

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Abstract

Actin purified from mouse fibroblasts by sodium dodecyl sulfate gel electrophoresis was used as antigen to obtain an antibody in rabbits. The elicited antibody was shown to be specific for actin as judged by immunodiffusion and complement fixation against partially purified mouse fibroblast actin and highly purified chicken muscle actin. The antibody was used in indirect immunofluorescence to demonstrate by fluorescence light microscopy the distribution and pattern of actin-containing filaments in a variety of cell types. Actin filaments were shown to span the cell length or to concentrate in "focal points" in patterns characteristic for each individual cell.


Item Type:Article
Additional Information:© 1974 by the National Academy of Sciences. Communicated by J. D. Watson, March 11, 1974. We thank Dr. F. Miller at Stonybrook University for the use of his animal facilities and his help in the preparation of the antibodies. We thank Dr. R. Pollack for the use of his laboratory; Drs. R. Goldman and C.-M. Chang and Art Vogel for their help with the fluorescent microscopy; and Drs. M. Osborn, C.W. Anderson, R. Goldman, and R. Pollack for their comments on the manuscript. We also thank Dr. Susan Lowey for a generous gift of purified chicken muscle actin. This investigation was supported by the National Institutes of Health (Research Grant CA-13106 from the National Cancer Institute).
Subject Keywords:immunofluorescence; microfilaments; sodium dodecyl sulfate gel electrophoresis
Record Number:CaltechAUTHORS:LAZpnas74a
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:LAZpnas74a
Alternative URL:http://dx.doi.org/10.1073/pnas.71.6.2268
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:7477
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:23 Feb 2007
Last Modified:14 Nov 2014 19:19

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