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Kinetics of expression of multiply spliced RNA in early human immunodeficiency virus type 1 infection of lymphocytes and monocytes

Klotman, Mary E. and Kim, Sunyoung and Buchbinder, Aby and DeRossi, Anita and Baltimore, David and Wong-Staal, Flossie (1991) Kinetics of expression of multiply spliced RNA in early human immunodeficiency virus type 1 infection of lymphocytes and monocytes. Proceedings of the National Academy of Sciences of the United States of America, 88 (11). pp. 5011-5015. ISSN 0027-8424. http://resolver.caltech.edu/CaltechAUTHORS:KLOpnas91

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Abstract

The genome of human immunodeficiency virus type 1 (HIV-1) encodes at least six proteins involved in regulation as well as the structural proteins Gag, Pol, and Env. The interplay of the various regulators generates early and late transcriptional phases in the HIV-1 life cycle; the earliest RNA is enriched in subgenomic species, and the genomic transcript appears at the later stage of infection. We investigated the nature of the mRNAs expressed in the early stages of infection when the 2 kilobase subgenomic species predominate. RNA was analyzed in the early phase of a one-step growth cycle of HIV-1 infection in T-lymphoid and monocytic cell lines by using PCR amplification of in vitro-synthesized viral cDNAs. In both cell lines, expression of Tat-, Rev-, and Nef-specific messages appeared simultaneously and could be detected within 8-12 hr of infection but in different amounts with a predominance of Nef-specific message. The Env-specific message could be detected as early as the Rev-specific message, indicating that expression of at least small amounts of the singly spliced message could occur before the accumulation of Rev.


Item Type:Article
Additional Information:© 1991 by the National Academy of Sciences. Contributed by David Baltimore, March 14, 1991. We acknowledge the continued support of Dr. Robert C. Gallo. M.E.K. is supported through the career development program of the Veteran's Administration Hospital. S.K. was a fellow of the Jane Coffin Childs Memorial Fund for Medical Research. This work is supported in part by Public Health Service Grants AI30897 (S.K.) and HL43510 (D.B.) and by the Clark Foundation for AIDS Research (S.K.). The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact.
Subject Keywords:alternative splicing; reverse transcriptase-PCR; regulatory genes; human retrovirus
Record Number:CaltechAUTHORS:KLOpnas91
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:KLOpnas91
Alternative URL:http://dx.doi.org/10.1073/pnas.88.11.5011
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:7600
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:12 Mar 2007
Last Modified:14 Nov 2014 19:19

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