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Blocked early-stage latency in the peripheral blood cells of certain individuals infected with human immunodeficiency virus type 1

Seshamma, Thikkavarapu and Bagasra, Omar and Trono, Didier and Baltimore, David and Pomerantz, Roger J. (1992) Blocked early-stage latency in the peripheral blood cells of certain individuals infected with human immunodeficiency virus type 1. Proceedings of the National Academy of Sciences of the United States of America, 89 (22). pp. 10663-10667. ISSN 0027-8424. http://resolver.caltech.edu/CaltechAUTHORS:SESpnas92

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Abstract

Human immunodeficiency virus type 1 (HIV-1) infections of humans have a natural history characterized by a variable but usually slow progression to an immunodeficient state. We have described a molecular model of HIV-1 proviral latency in certain cell lines, characterized by extremely low or undetectable levels of unspliced genomic HIV-1-specific RNA but significant levels of multiply spliced HIV-1-specific RNA. We have utilized a quantitative reverse transcriptase-initiated polymerase chain reaction to measure the levels of various HIV-1 RNA species in peripheral blood mononuclear cells. The median level of multiply spliced HIV-1 RNA was dramatically higher than the median level of unspliced viral RNA in asymptomatic individuals. In addition, HIV-1 RNA patterns characterized by at least a 10-fold excess of multiply spliced to unspliced viral RNA were significantly more common in asymptomatic individuals than in patients with the acquired immunodeficiency syndrome. We suggest that asymptomatic clinical HIV-1 infection is characterized by a preponderance of HIV-1-infected peripheral blood cells blocked at an early stage of HIV-1 infection. This viral expression pattern, which we have called blocked early-stage latency, may constitute a reservoir of latently infected cells in certain HIV-1-infected persons.


Item Type:Article
Additional Information:© 1992 by the National Academy of Sciences. Contributed by David Baltimore, July 22, 1992. We thank Joseph Oakes for technical assistance; Dr. Stephen Hauptman, Dr. Mark Sachs, Ms. Lisa Creran, Ms. Roberta Benjamin, and Ms. Joann Stockman for their help in obtaining patient samples; Dr. Hyman Menduke for assistance with statistical analyses; and Ms. Rita Victor and Ms. Brenda Gordon for secretarial assistance. This work was supported in part by U.S. Public Health Service Awards AI00930 and AI31836 and a grant from the W.W. Smith Charitable Trust. The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact.
Subject Keywords:T-CELL; EXPRESSION; HIV-1; RNA; PATHOGENESIS; REPLICATION; MECHANISMS
Record Number:CaltechAUTHORS:SESpnas92
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:SESpnas92
Alternative URL:http://dx.doi.org/10.1073/pnas.89.22.10663
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:7612
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:14 Mar 2007
Last Modified:26 Dec 2012 09:33

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