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Establishment of tight junctions between epithelial cells

Hudspeth, A. J. (1975) Establishment of tight junctions between epithelial cells. Proceedings of the National Academy of Sciences of the United States of America, 72 (7). pp. 2711-2713. ISSN 0027-8424. http://resolver.caltech.edu/CaltechAUTHORS:HUDpnas75

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Abstract

Epithelia serve as barriers to the diffusion of solutes between body compartments, and must do so despite the frequent loss of cells. When single cells are experimentally removed from the Necturus gallbladder epithelium, contiguous cells migrate to fill the defect within 30 min. Electrophysiological measurements show that the local electrical resistance across the epithelium in the region of a wound returns to normal in the same period of time; electron microscopy demonstrates that tight junctions are formed concurrently. Physiologically functional and morphologically recognizable tight junctions can thus be established within 30 min, demonstrating a mechanism for the rapid restoration of epithelial integrity after cell loss.


Item Type:Article
Additional Information:© 1975 by the National Academy of Sciences. Communicated by Stephen W. Kuffler, April 22, 1975. I thank Drs. B. Forslind, E.A. Kravitz, U.J. McMahan, and T.N. Wiesel for the use of equipment, Dr. A.G. Yee for assistance in freeze-fracturing, and Drs. S.W. Kuffler, E. Raviola, and A.E. Stuart for comments on the manuscript. This research was supported in part by USPHS Grant MH-07084 and by a fellowship from the Alfred P. Sloan Foundation.
Subject Keywords:electron microscopy; electrophysiology; intercellular contacts; membranes; wound healing
Record Number:CaltechAUTHORS:HUDpnas75
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:HUDpnas75
Alternative URL:http://dx.doi.org/10.1073/pnas.72.7.2711
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:7619
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:14 Mar 2007
Last Modified:26 Dec 2012 09:33

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